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Literature summary extracted from

  • Saburi, W.; Mori, H.; Saito, S.; Okuyama, M.; Kimura, A.
    Structural elements in dextran glucosidase responsible for high specificity to long chain substrate (2006), Biochim. Biophys. Acta, 1764, 688-698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.70 wild-type and mutant enzymes produced in Escherichia coli Streptococcus mutans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.70 W238A mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose Streptococcus mutans
3.2.1.70 W238N mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose Streptococcus mutans
3.2.1.70 W238P mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.70 0.549
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 1.03
-
 panose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 2.2
-
 panose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 3.17
-
 isomaltotriose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 4.22
-
 panose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 4.96
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 5.1
-
 isomaltopentaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 5.58
-
 isomaltotetraose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 6.41
-
 panose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 7.77
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 7.88
-
 isomaltohexaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 8.91
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 9.35
-
 isomaltose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 9.92
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 10.2
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 11.4
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 12
-
 isomaltoheptaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 15.1
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 15.8
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 16.1
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 19
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 19.9
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 20.5
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 20.9
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 24.6
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 26.9
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 27.1
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 38.3
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 41.4
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 43.6
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 76.9
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 81.7
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.70 63000
-
 wild-type enzyme, gel filtration Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.70 Streptococcus mutans Q2HWU5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.70 4-nitrophenyl alpha-D-glucoside + H2O 377% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 4-nitrophenol + D-glucose
-
 ?
3.2.1.70 dextran + H2O
-
 Streptococcus mutans alpha-D-glucose
-
 ?
3.2.1.70 isomaltoheptaose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
3.2.1.70 isomaltohexaose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
3.2.1.70 isomaltopentaose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
3.2.1.70 isomaltose + H2O
-
 Streptococcus mutans 2 D-glucose
-
 ?
3.2.1.70 isomaltotetraose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
3.2.1.70 isomaltotriose + H2O 221% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
3.2.1.70 kojibiose + H2O 4.02% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 2 D-glucose
-
 ?
3.2.1.70 maltose + H2O 0.0135% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 2 D-glucose
-
 ?
3.2.1.70 maltotetraose + H2O 0.0108% of the activity with isomaltose, wild-type enzyme Streptococcus mutans maltotriose + D-glucose
-
 ?
3.2.1.70 maltotriose + H2O 0.146% of the activity with isomaltose, wild-type enzyme Streptococcus mutans maltose + D-glucose
-
 ?
3.2.1.70 additional information no activity with trehalose Streptococcus mutans ?
-
 ?
3.2.1.70 nigerose + H2O 1.99% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 2 D-glucose
-
 ?
3.2.1.70 panose + H2O 417% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
3.2.1.70 sucrose + H2O 4.29% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.70 monomer 1 * 63000, wild-type enzyme, SDS-PAGE Streptococcus mutans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.70 dextran glucosidase
-
 Streptococcus mutans
3.2.1.70 SMDG
-
 Streptococcus mutans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.70 40
-
 15 min, pH 6.0, stable below Streptococcus mutans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.70 0.04 1.97 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 0.04 1.97 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 3 6 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 7.8
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 8.88
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 8.96
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 9.21
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 9.81
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 12.2
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 22.3
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 22.4
-
 panose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 22.6
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 33.1
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 33.2
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 34.5
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 39.6
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 42.1
-
 panose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 42.4
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 52.5
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 56.5
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 61.4
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.2.1.70 77.5
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 79.8
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 121
-
 panose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
3.2.1.70 202
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
3.2.1.70 235
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 401
-
 isomaltohexaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 419
-
 isomaltopentaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 449
-
 isomaltoheptaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 483
-
 isomaltose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 499
-
 isomaltotetraose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 538
-
 panose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 541
-
 isomaltotriose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
3.2.1.70 1202
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.70 6
-
-
 Streptococcus mutans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.70 5.5 8.4 4°C, stable for 18 h Streptococcus mutans