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Literature summary extracted from

  • Nagahama, M.; Otsuka, A.; Sakurai, J.
    Role of tyrosine-57 and -65 in membrane-damaging and sphingomyelinase activities of Clostridium perfringens alpha-toxin (2006), Biochim. Biophys. Acta, 1762, 110-114.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 H148G mutant enzyme shows no sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y57A mutant enzyme shows 111.3% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y57C/C169L mutant enzyme shows 104.1% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y57F mutant enzyme shows 105.2% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y57L mutant enzyme shows 103.1% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y65A mutant enzyme shows 89.4% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y65C/C169L mutant enzyme shows 98.1% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y65F mutant enzyme shows 95.6% of wild-type sphingomyelinase activity Clostridium perfringens
3.1.4.12 Y65L mutant enzyme shows 99.2% of wild-type sphingomyelinase activity Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 wild-type and mutant enzymes Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Clostridium perfringens N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 alpha-toxin
-
 Clostridium perfringens