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Literature summary extracted from

  • Yamashita, H.; Itsuki, A.; Kimoto, M.; Hiemori, M.; Tsuji, H.
    Acetate generation in rat liver mitochondria: acetyl-CoA hydrolase activity is demonstrated by 3-ketoacyl-CoA thiolase (2006), Biochim. Biophys. Acta, 1761, 17-23.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.16 additional information di-(2-ethylhexyl)phthalate induces the enzyme Rattus norvegicus
3.1.2.1 additional information the enzyme is induced in mitochondria by di(2-ethylhexyl)phthalate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.16 acetoacetyl-CoA competitive inhibition of the acetyl-CoA hydrolase activity Rattus norvegicus
2.3.1.16 acetyl-CoA competitive inhibition of the 3-ketoacyl-CoA thiolase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.16 additional information
-
 additional information kinetics Rattus norvegicus
2.3.1.16 0.0114
-
 acetoacetyl-CoA pH 7.4 Rattus norvegicus
2.3.1.16 0.71
-
 acetyl-CoA pH 7.4 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.16 mitochondrion
-
 Rattus norvegicus 5739
-
3.1.2.1 mitochondrion exclusively Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.1 43000
-
 x * 43000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.16 acetyl-CoA + H2O Rattus norvegicus 3-ketoacyl-CoA thiolase activity acetate + CoA
-
 ?
2.3.1.16 additional information Rattus norvegicus the enzyme is involved in fatty acid beta-oxidation ?
-
 ?
3.1.2.1 acetyl-CoA + H2O Rattus norvegicus
-
 CoA + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.16 Rattus norvegicus
-
-
-
3.1.2.1 Rattus norvegicus
-
 male Spargue-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.16 native enzyme 27.2fold from liver mitochondria preparation by 5 steps of ion exchange and hydrophobic interaction chromatography Rattus norvegicus
3.1.2.1 27.2fold from liver mitochondria in a 5-step chromatographical procedure Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.1 acetyl-CoA + H2O = CoA + acetate the bifunctional enzyme is identical with a 3-ketoacyl-CoA thiolase isozyme, i.e. THL, EC 2.3.1.9, determined by internal peptide amino acid sequence comparison Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.16 brown adipose tissue di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
2.3.1.16 heart di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
2.3.1.16 intestine di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
2.3.1.16 kidney di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
2.3.1.16 liver di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
3.1.2.1 brown adipose tissue
-
 Rattus norvegicus
-
3.1.2.1 heart
-
 Rattus norvegicus
-
3.1.2.1 intestine
-
 Rattus norvegicus
-
3.1.2.1 kidney
-
 Rattus norvegicus
-
3.1.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.16 0.543
-
 purified enzyme, acetyl-CoA hydrolase activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.16 acetyl-CoA + H2O 3-ketoacyl-CoA thiolase activity Rattus norvegicus acetate + CoA
-
 ?
2.3.1.16 acetyl-CoA + H2O best acyl-CoA substrate, acetyl-CoA hydrolase activity Rattus norvegicus acetate + CoA
-
 ?
2.3.1.16 acetyl-CoA + H2O preferred substrate, 3-ketoacyl-CoA thiolase activity Rattus norvegicus acetate + CoA
-
 ?
2.3.1.16 butyryl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus butanoate + CoA
-
 ?
2.3.1.16 dodecanoyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus dodecanoate + CoA
-
 ?
2.3.1.16 hexanoyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus hexanoate + CoA
-
 ?
2.3.1.16 additional information the enzyme is involved in fatty acid beta-oxidation Rattus norvegicus ?
-
 ?
2.3.1.16 additional information analysis of the substrate chain length specificity of the enzyme, high preference for acetyl-CoA Rattus norvegicus ?
-
 ?
2.3.1.16 palmitoyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus palmitate + CoA
-
 ?
2.3.1.16 propionyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus propionate + CoA
-
 ?
3.1.2.1 acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
3.1.2.1 acetyl-CoA + H2O highly preferred substrate Rattus norvegicus CoA + acetate
-
 ?
3.1.2.1 additional information substrate and chain-length specificity of the mitochondrial enzyme, overview Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.1 ? x * 43000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.16 3-ketoacyl-CoA thiolase
-
 Rattus norvegicus
3.1.2.1 ACH
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.1 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.16 7.4
-
 assay at Rattus norvegicus
3.1.2.1 8.3
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.16 acetyl-CoA
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.16 0.0022
-
 acetoacetyl-CoA pH 7.4, competitive versus acetyl-CoA hydrolysis Rattus norvegicus
2.3.1.16 0.24
-
 acetyl-CoA pH 7.4, competitive versus 3-ketoacyl-CoA thiolase activity Rattus norvegicus