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Literature summary extracted from

  • Goda, S.; Sakuraba, H.; Kawarabayasi, Y.; Ohshima, T.
    The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization (2005), Biochim. Biophys. Acta, 1748, 110-115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.11 expression in Escherichia coli. Mainly obtained as inactive inclusion body in Escherichia coli Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.11 putrescine
-
 Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.11 0.53
-
 agmatine 50°C, pH 11.3 Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.11 Ca2+ enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2 Pyrococcus horikoshii
3.5.3.11 Co2+ enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2 Pyrococcus horikoshii
3.5.3.11 Mg2+ enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2 Pyrococcus horikoshii
3.5.3.11 Mn2+ enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2 Pyrococcus horikoshii
3.5.3.11 Zn2+ enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2 Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.11 31000
-
 4 * 31000, SDS-PAGE Pyrococcus horikoshii
3.5.3.11 145000
-
 gel filtration Pyrococcus horikoshii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.3.11 dimethyl sulfoxide 10%, no loss of activity at 50°C Pyrococcus horikoshii
3.5.3.11 Ethanol 10%, no loss of activity at 50°C Pyrococcus horikoshii
3.5.3.11 Methanol 10%, no loss of activity at 50°C Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.11 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.11 recombinant Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.11 agmatine + H2O
-
 Pyrococcus horikoshii putrescine + urea
-
 ?
3.5.3.11 additional information no activity with arginine Pyrococcus horikoshii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.11 tetramer 4 * 31000, SDS-PAGE Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.11 100
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.11 80
-
 10 min, stable Pyrococcus horikoshii
3.5.3.11 90
-
 10 min, 53% loss of activity Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.11 11
-
-
 Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.11 9.5 12.5 pH 9.5: about 50% of maximal activity, pH 12.5: about 85% of maximal activity Pyrococcus horikoshii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.11 1.8
-
 putrescine 50°C, pH 11.3 Pyrococcus horikoshii