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Literature summary extracted from

  • Raychaudhuri, A.; Jerga, A.; Tipton, P.A.
    Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa (2005), Biochemistry, 44, 2974-2981.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.184 gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.184 0.0059
-
 butyryl-[acyl-carrier protein 2] pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.3.1.184 0.0074
-
 butyryl-[acyl-carrier protein 1] pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.3.1.184 0.2
-
 butyryl-CoA pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.3.1.184 0.283
-
 butyryl-[acyl-carrier protein 3] pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.184 Mg2+
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] Pseudomonas aeruginosa substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.184 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.184 recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.184 an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone structures of substrate, product, and transition state, overview Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.184 S-adenosyl-L-methionine + butyryl-CoA substrate of RhlI, very low activity Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 1] substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 1]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 2] substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 2]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 3] substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates, very low activity with ACP3 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 3]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.184 acylhomoserine lactone synthase
-
 Pseudomonas aeruginosa
2.3.1.184 RhlI
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.184 25
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.184 0.026
-
 butyryl-[acyl-carrier protein 3] pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.3.1.184 0.05
-
 butyryl-CoA pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.3.1.184 0.35
-
 butyryl-[acyl-carrier protein 1] pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.3.1.184 0.46
-
 butyryl-[acyl-carrier protein 2] pH 8.8, 25°C, recombinant enzyme Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.184 8.8
-
 assay at Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.184 additional information
-
 pH-profile Pseudomonas aeruginosa