EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.18 | - |
Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.18 | E138A | inactive mutant enzyme | Pseudomonas putida |
4.2.1.18 | E138D | the ratio of turnover number to KM-value is 9fold lower than the wild-type ratio | Pseudomonas putida |
4.2.1.18 | E138Q | the ratio of turnover number to KM-value is 3600fold lower than the wild-type ratio | Pseudomonas putida |
4.2.1.18 | G118E | no activity with crotonyl-CoA, methylcrotonyl-CoA and methylglutaconyl-CoA | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.18 | 3-methylcrotonyl-CoA | - |
Pseudomonas putida | |
4.2.1.18 | crotonyl-CoA | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.18 | 0.0291 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, wild-type enzyme | Pseudomonas putida | |
4.2.1.18 | 0.043 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138Q | Pseudomonas putida | |
4.2.1.18 | 0.08 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138D | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.18 | Pseudomonas putida | - |
PA2011 | - |
4.2.1.18 | Pseudomonas putida PA2011 | - |
PA2011 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.18 | - |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.18 | additional information | no activity with crotonyl-CoA and methylcrotonyl-CoA | Pseudomonas putida | ? | - |
? | |
4.2.1.18 | additional information | no activity with crotonyl-CoA and methylcrotonyl-CoA | Pseudomonas putida PA2011 | ? | - |
? | |
4.2.1.18 | trans-3-methylglutaconyl-CoA + H2O | - |
Pseudomonas putida | (S)-3-hydroxymethylglutaryl-CoA | - |
? | |
4.2.1.18 | trans-3-methylglutaconyl-CoA + H2O | - |
Pseudomonas putida PA2011 | (S)-3-hydroxymethylglutaryl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.18 | (3S)-methylglutaconyl-CoA hydratase | - |
Pseudomonas putida |
4.2.1.18 | MGCH | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.18 | 0.00086 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138Q | Pseudomonas putida | |
4.2.1.18 | 0.064 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138D | Pseudomonas putida | |
4.2.1.18 | 2.1 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, wild-type enzyme | Pseudomonas putida |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.18 | 0.0317 | - |
3-methylcrotonyl-CoA | pH 7.09, 25°C, wild-type enzyme | Pseudomonas putida | |
4.2.1.18 | 0.0949 | - |
crotonyl-CoA | pH 7.9, 25°C, wild-type enzyme | Pseudomonas putida |