Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wong, B.J.; Gerlt, J.A.
    Evolution of function in the crotonase superfamily: (3S)-methylglutaconyl-CoA hydratase from Pseudomonas putida (2004), Biochemistry, 43, 4646-4654.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.18
-
Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.18 E138A inactive mutant enzyme Pseudomonas putida
4.2.1.18 E138D the ratio of turnover number to KM-value is 9fold lower than the wild-type ratio Pseudomonas putida
4.2.1.18 E138Q the ratio of turnover number to KM-value is 3600fold lower than the wild-type ratio Pseudomonas putida
4.2.1.18 G118E no activity with crotonyl-CoA, methylcrotonyl-CoA and methylglutaconyl-CoA Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.18 3-methylcrotonyl-CoA
-
Pseudomonas putida
4.2.1.18 crotonyl-CoA
-
Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.18 0.0291
-
trans-3-methylglutaconyl-CoA pH 7.9, 25°C, wild-type enzyme Pseudomonas putida
4.2.1.18 0.043
-
trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138Q Pseudomonas putida
4.2.1.18 0.08
-
trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138D Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.18 Pseudomonas putida
-
PA2011
-
4.2.1.18 Pseudomonas putida PA2011
-
PA2011
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.18
-
Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.18 additional information no activity with crotonyl-CoA and methylcrotonyl-CoA Pseudomonas putida ?
-
?
4.2.1.18 additional information no activity with crotonyl-CoA and methylcrotonyl-CoA Pseudomonas putida PA2011 ?
-
?
4.2.1.18 trans-3-methylglutaconyl-CoA + H2O
-
Pseudomonas putida (S)-3-hydroxymethylglutaryl-CoA
-
?
4.2.1.18 trans-3-methylglutaconyl-CoA + H2O
-
Pseudomonas putida PA2011 (S)-3-hydroxymethylglutaryl-CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.18 (3S)-methylglutaconyl-CoA hydratase
-
Pseudomonas putida
4.2.1.18 MGCH
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.18 0.00086
-
trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138Q Pseudomonas putida
4.2.1.18 0.064
-
trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138D Pseudomonas putida
4.2.1.18 2.1
-
trans-3-methylglutaconyl-CoA pH 7.9, 25°C, wild-type enzyme Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.18 0.0317
-
3-methylcrotonyl-CoA pH 7.09, 25°C, wild-type enzyme Pseudomonas putida
4.2.1.18 0.0949
-
crotonyl-CoA pH 7.9, 25°C, wild-type enzyme Pseudomonas putida