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Literature summary extracted from

  • Kruger, R.G.; Otvos, B.; Frankel, B.A.; Bentley, M.; Dostal, P.; McCafferty, D.G.
    Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA (2004), Biochemistry, 43, 1541-1551.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.70 medicine SrtA activity is a prime target for inhibition of Staphylococcus aureus colonization Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 expression of SrtADELTAN24 in a His6-tagged form Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.70 additional information SrtA activity is a prime target for inhibition of Staphylococcus aureus colonization Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.70 0.137
-
 Gly
-
 Staphylococcus aureus
3.4.22.70 5.5
-
 Abz-LPETG-Dap(Dnp)-NH2
-
 Staphylococcus aureus
3.4.22.70 5.6
-
 Dnp-AQALPETGEE-NH2
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.70 additional information Staphylococcus aureus primary role of the SrtA isoform in Staphylococcus aureus adhesion and host colonization ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 recombinant SrtADELTAN24 in a His6-tagged form Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.70 0.0255
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 Abz-LPETG-Dap(Dnp)-NH2 + Gly5
-
 Staphylococcus aureus Abz-LPETGGGGG-OH + Gly-Dap(Dnp)-NH2
-
 ?
3.4.22.70 Dnp-AQALPETGEE-NH2 + Gly5
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 additional information primary role of the SrtA isoform in Staphylococcus aureus adhesion and host colonization Staphylococcus aureus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.70 0.013
-
 Dnp-AQALPETGEE-NH2
-
 Staphylococcus aureus
3.4.22.70 0.27
-
 Abz-LPETG-Dap(Dnp)-NH2
-
 Staphylococcus aureus