EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | 3-indoleethanol | 1 mM, enhances activity by 87% | Oryctolagus cuniculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | 3-indoleethanol | lowers Km value for D-tryptophan by 25% at pH 7, enhances Vmax by 40-60% | Oryctolagus cuniculus | |
1.13.11.52 | azide | - |
Oryctolagus cuniculus | |
1.13.11.52 | CO | - |
Oryctolagus cuniculus | |
1.13.11.52 | cyanide | heme ligand, competitive for the ferric enzyme | Oryctolagus cuniculus | |
1.13.11.52 | indole | lowers Km value for D-tryptophan by 60% at pH 7, lowers Vmax by 12-24%, at 1 mM lowers activity by 13% | Oryctolagus cuniculus | |
1.13.11.52 | additional information | 3-indoleethanol and indole affect the L-tryptophan affinity of the ferrous enzyme, indole enhances affinity of enzyme for cyanide and azide and norharman, L-tryptophan lowers norharman affinity for the ferrous dioxygenase | Oryctolagus cuniculus | |
1.13.11.52 | NO | - |
Oryctolagus cuniculus | |
1.13.11.52 | norharman | can bind to the ferric and ferrous enzyme to from a quarternary complex | Oryctolagus cuniculus | |
1.13.11.52 | O2 | - |
Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.52 | 0.005 | - |
L-tryptophan | pH 6.0 | Oryctolagus cuniculus | |
1.13.11.52 | 0.66 | - |
D-tryptophan | pH 7.0, 1 mM indole | Oryctolagus cuniculus | |
1.13.11.52 | 1.3 | - |
D-tryptophan | pH 7.0, 1 mM 3-indoleethanol | Oryctolagus cuniculus | |
1.13.11.52 | 1.7 | - |
D-tryptophan | pH 7.0 | Oryctolagus cuniculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.52 | D-tryptophan + O2 | Oryctolagus cuniculus | - |
N-formyl-D-kynurenine | - |
? | |
1.13.11.52 | L-tryptophan + O2 | Oryctolagus cuniculus | - |
N-formyl-L-kynurenine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.52 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.13.11.52 | highly autoxidizable, requires superoxide anion or a reductant such as ascorbic acid and methylene blue as cofactors | Oryctolagus cuniculus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.52 | - |
Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.52 | small intestine | - |
Oryctolagus cuniculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.52 | D-tryptophan + O2 | - |
Oryctolagus cuniculus | N-formyl-D-kynurenine | - |
? | |
1.13.11.52 | L-tryptophan + O2 | - |
Oryctolagus cuniculus | N-formyl-L-kynurenine | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.52 | additional information | ascorbic acid in combination with methylene blue or toluidine blue | Oryctolagus cuniculus | |
1.13.11.52 | additional information | superoxide anion in combination with methylene blue or toluidine blue | Oryctolagus cuniculus |