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Literature summary extracted from

  • Sono, M.
    Enzyme kinetic and spectroscopic studies of inhibitor and effector interactions with indoleamine 2,3-dioxygenase. 2. Evidence for the existence of another binding site in the enzyme for indole derivative effectors (1989), Biochemistry, 28, 5400-5407.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 3-indoleethanol 1 mM, enhances activity by 87% Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 3-indoleethanol lowers Km value for D-tryptophan by 25% at pH 7, enhances Vmax by 40-60% Oryctolagus cuniculus
1.13.11.52 azide
-
Oryctolagus cuniculus
1.13.11.52 CO
-
Oryctolagus cuniculus
1.13.11.52 cyanide heme ligand, competitive for the ferric enzyme Oryctolagus cuniculus
1.13.11.52 indole lowers Km value for D-tryptophan by 60% at pH 7, lowers Vmax by 12-24%, at 1 mM lowers activity by 13% Oryctolagus cuniculus
1.13.11.52 additional information 3-indoleethanol and indole affect the L-tryptophan affinity of the ferrous enzyme, indole enhances affinity of enzyme for cyanide and azide and norharman, L-tryptophan lowers norharman affinity for the ferrous dioxygenase Oryctolagus cuniculus
1.13.11.52 NO
-
Oryctolagus cuniculus
1.13.11.52 norharman can bind to the ferric and ferrous enzyme to from a quarternary complex Oryctolagus cuniculus
1.13.11.52 O2
-
Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.005
-
L-tryptophan pH 6.0 Oryctolagus cuniculus
1.13.11.52 0.66
-
D-tryptophan pH 7.0, 1 mM indole Oryctolagus cuniculus
1.13.11.52 1.3
-
D-tryptophan pH 7.0, 1 mM 3-indoleethanol Oryctolagus cuniculus
1.13.11.52 1.7
-
D-tryptophan pH 7.0 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.52 D-tryptophan + O2 Oryctolagus cuniculus
-
N-formyl-D-kynurenine
-
?
1.13.11.52 L-tryptophan + O2 Oryctolagus cuniculus
-
N-formyl-L-kynurenine
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Oryctolagus cuniculus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.52 highly autoxidizable, requires superoxide anion or a reductant such as ascorbic acid and methylene blue as cofactors Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52
-
Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.52 small intestine
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 D-tryptophan + O2
-
Oryctolagus cuniculus N-formyl-D-kynurenine
-
?
1.13.11.52 L-tryptophan + O2
-
Oryctolagus cuniculus N-formyl-L-kynurenine
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 additional information ascorbic acid in combination with methylene blue or toluidine blue Oryctolagus cuniculus
1.13.11.52 additional information superoxide anion in combination with methylene blue or toluidine blue Oryctolagus cuniculus