Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
    Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures (2004), Biochem. J., 377, 419-428.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 additional information phosphate starvation induces the enzyme activity 4fold Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Cu2+ 99% inhibition at 10 mM Solanum lycopersicum
3.1.3.2 Fe3+ complete inhibition at 10 mM Solanum lycopersicum
3.1.3.2 fluoride mixed type Solanum lycopersicum
3.1.3.2 molybdate competitive Solanum lycopersicum
3.1.3.2 additional information the purple enzyme is insensitive to L-tartrate Solanum lycopersicum
3.1.3.2 phosphate mixed type Solanum lycopersicum
3.1.3.2 vanadate mixed type Solanum lycopersicum
3.1.3.2 Zn2+ 88% inhibition at 10 mM Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.7 intracellular
-
 Solanum lycopersicum 5622
-
3.1.3.2 intracellular
-
 Solanum lycopersicum 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.7 additional information the peroxidase activity of the enzyme is insensitive to Mg2+, Zn2+, phosphate, and molydan Solanum lycopersicum
3.1.3.2 Mg2+ activates 150% at 10 mM Solanum lycopersicum
3.1.3.2 Mn2+ activates 130% at 10 mM Solanum lycopersicum
3.1.3.2 additional information the enzyme is not affected by BaCl2, CaCl2, CoCl2, KCl, NaCl, or EDTA Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 57000
-
 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
1.11.1.7 63000
-
 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
1.11.1.7 142000
-
 native PAGE Solanum lycopersicum
3.1.3.2 57000
-
 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
3.1.3.2 63000
-
 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
3.1.3.2 142000
-
 native PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.2 additional information Solanum lycopersicum the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Solanum lycopersicum
-
-
-
3.1.3.2 Solanum lycopersicum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.7 glycoprotein
-
 Solanum lycopersicum
3.1.3.2 glycoprotein
-
 Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography Solanum lycopersicum
3.1.3.2 intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 cell culture phosphate-starved Solanum lycopersicum
-
3.1.3.2 cell culture phosphate-starved Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 10
-
 purified enzyme Solanum lycopersicum
3.1.3.2 620
-
 purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 5-aminophthalhydrazide + H2O2 i.e. luminol, chemiluminescent substrate Solanum lycopersicum ?
-
 ?
1.11.1.7 additional information substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits also acid phosphatase, EC 3.1.3.2 Solanum lycopersicum ?
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Solanum lycopersicum 4-nitrophenol + phosphate
-
 ?
3.1.3.2 additional information the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism Solanum lycopersicum ?
-
 ?
3.1.3.2 additional information substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits peroxidase activity, EC 1.11.1.7, optimal at pH 9.0 and insensitive to Mg2+ and molydate Solanum lycopersicum ?
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O best substrate Solanum lycopersicum pyruvate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 dimer 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
1.11.1.7 More peptide mapping Solanum lycopersicum
3.1.3.2 dimer 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE Solanum lycopersicum
3.1.3.2 More peptide mapping Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 IAP
-
 Solanum lycopersicum
3.1.3.2 intracellular acid phosphatase
-
 Solanum lycopersicum
3.1.3.2 PAP
-
 Solanum lycopersicum
3.1.3.2 purple acid phosphatase
-
 Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 additional information
-
-
 Solanum lycopersicum
3.1.3.2 50
-
 loss of 20% activity after 5 min Solanum lycopersicum
3.1.3.2 65
-
 loss of 50% activity after 5 min Solanum lycopersicum
3.1.3.2 70
-
 loss of 80% activity after 5 min Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 9
-
-
 Solanum lycopersicum
3.1.3.2 5.1
-
-
 Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4.8 6.5
-
 Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0002
-
 molybdate pH 5.1 Solanum lycopersicum
3.1.3.2 0.0065
-
 vanadate pH 5.1 Solanum lycopersicum
3.1.3.2 0.094
-
 fluoride pH 5.1 Solanum lycopersicum
3.1.3.2 1.6
-
 phosphate pH 5.1 Solanum lycopersicum