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Literature summary extracted from

  • Watanabe, Y.; Fujiwara, M.; Yoshida, R.; Hayaishi, O.
    Stereospecificity of hepatic L-tryptophan 2,3-dioxygenase (1980), Biochem. J., 189, 393-405.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 5-hydroxy-D-tryptophan 0.1 mM, 26% inhibition of cleavage of D-tryptophan, 29% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 5-hydroxy-L-tryptophan 0.1 mM, 40% inhibition of cleavage of D-tryptophan, 53% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 indole-3-acetic acid 1 mM, 19% inhibition of cleavage of D-tryptophan, 27% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 indole-3-acrylic acid competitive inhibitor Mus musculus
1.13.11.52 iodoacetic acid 1 mM, 12% inhibition of cleavage of D-tryptophan, 7% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 KCN 0.01 mM, 39% inhibition of cleavage of D-tryptophan, 48% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 additional information not inhibited by superoxide dismutase Mus musculus
1.13.11.52 N-ethylmaleimide 1 mM, 58% inhibition of cleavage of D-tryptophan, 45% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 NaN3 10 mM, 33% inhibition of cleavage of D-tryptophan, 32% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 serotonin 1 mM, 36% inhibition of cleavage of D-tryptophan, 37% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 Tiron 10 mM, 2% inhibition of cleavage of D-tryptophan, 6% inhibition of cleavage of L-tryptophan Mus musculus
1.13.11.52 tryptamine 0.1 mM, 16% inhibition of cleavage of D-tryptophan, 11% inhibition of cleavage of L-tryptophan Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.21
-
 L-tryptophan
-
 Mus musculus
1.13.11.52 0.96
-
 D-tryptophan
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.52 150000
-
 alpha2beta2 subunit Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Bos taurus
-
-
-
1.13.11.52 Capra hircus
-
-
-
1.13.11.52 Homo sapiens
-
-
-
1.13.11.52 Macaca fuscata fuscata
-
-
-
1.13.11.52 Mus musculus
-
-
-
1.13.11.52 Oryctolagus cuniculus
-
-
-
1.13.11.52 Pseudomonas fluorescens
-
-
-
1.13.11.52 Rattus norvegicus
-
 Sprague Dawley
-
1.13.11.52 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52 ammonium sulfate precipitation, hydroxyapatite column chromatography (two times), gel filtration, DEAE cellulose column chromatography, third hydroxyapatite column chromatography, Sephadex G-200 gel filtration, second DEAE cellulose column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.52 liver
-
 Mus musculus
-
1.13.11.52 liver
-
 Homo sapiens
-
1.13.11.52 liver
-
 Rattus norvegicus
-
1.13.11.52 liver
-
 Sus scrofa
-
1.13.11.52 liver
-
 Bos taurus
-
1.13.11.52 liver
-
 Oryctolagus cuniculus
-
1.13.11.52 liver
-
 Capra hircus
-
1.13.11.52 liver
-
 Macaca fuscata fuscata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.52 0.55
-
 25°C, 50 mM HEPES/NaOH (pH 7.3), 5 mM D-tryptophan 0.01 mM methylene blue, 5 mM ascorbic acid, 0.001 mM catalase Mus musculus
1.13.11.52 2.13
-
 25°C, 50 mM HEPES/NaOH (pH 7.3), 2 mM L-tryptophan 0.01 mM methylene blue, 5 mM ascorbic acid, 0.001 mM catalase Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 D-tryptophan + O2
-
 Mus musculus N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Mus musculus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Rattus norvegicus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Sus scrofa N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Bos taurus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Oryctolagus cuniculus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Pseudomonas fluorescens N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Capra hircus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Macaca fuscata fuscata N-formyl-L-kynurenine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.52 7.3
-
 25°C Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.52 0.0045
-
 indole-3-acrylic acid against L-tryptophan Mus musculus
1.13.11.52 0.0068
-
 indole-3-acrylic acid against D-tryptophan Mus musculus