Literature summary extracted from
Murakami, M.T.; Fernandes-Pedrosa, M.F.; de Andrade, S.A.; Gabdoulkhakov, A.; Betzel, C.; Tambourgi, D.V.; Arni, R.K.
Structural insights into the catalytic mechanism of sphingomyelinases D and evolutionary relationship to glycerophosphodiester phosphodiesterases (2006), Biochem. Biophys. Res. Commun., 342, 323-329.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.4.41 |
hanging-drop vapour-diffusion method. Crystal structure of the sulfate free enzyme determined at 1.85 A resolution. The metal ion is tetrahedrally coordinated instead of the trigonal-bipyramidal coordination observed in the sulfate bound form |
Loxosceles laeta |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.4.41 |
Mg2+ |
Mg2+-ion-dependent acid-base mechanism which involves two histidines |
Loxosceles laeta |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.4.41 |
Loxosceles laeta |
Q8I914 |
recombinant |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.4.41 |
venom |
- |
Loxosceles laeta |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.4.41 |
sphingomyelin + H2O |
Mg2+-ion-dependent acid-base mechanism which involves two histidines |
Loxosceles laeta |
choline + ceramide phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.4.41 |
SMase I |
- |
Loxosceles laeta |