Literature summary extracted from
Czaja, R.; Perbandt, M.; Betzel, C.; Hahn, U.
Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine (2005), Biochem. Biophys. Res. Commun., 336, 882-889.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.6.1.24 |
crystal structure of RNase T1-T2 (K41E/Y42F/N43R/Y45W/E46N/W59Y), hanging drop vapor diffusion method, 2.1 A resolution |
Aspergillus oryzae |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.6.1.24 |
K41E/Y42F/N43R/Y45W/E46N/W59Y |
RNase T1-R2 contains the additional mutation W59Y compared to RNase T1-RV variant. RNase T1-RV is a variant where the specificity is changed from guanine to purine, accompanied with a reduced activity. The additional mutation W59Y increases the activity in comparison to variant RV to 425% |
Aspergillus oryzae |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.6.1.24 |
additional information |
- |
additional information |
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) |
Aspergillus oryzae |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.24 |
Aspergillus oryzae |
P00651 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.6.1.24 |
adenylyl-3',5'-cytidine + H2O |
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) |
Aspergillus oryzae |
adenosine 3'-phosphate + cytidine |
- |
? |
|
4.6.1.24 |
guanylyl-3',5'-cytidine + H2O |
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) |
Aspergillus oryzae |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.6.1.24 |
RNase T1-R2 |
mutant RNase T1 K41E/Y42F/N43R/Y45W/E46N/W59Y |
Aspergillus oryzae |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
4.6.1.24 |
additional information |
- |
additional information |
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) |
Aspergillus oryzae |
|