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Literature summary extracted from

  • Asano, Y.; Kato, Y.; Levy, C.; Baker, P.; Rice, D.
    Structure and Function of Amino Acid Ammonia-lyases (2004), Biocatal. Biotransform., 22, 131-138.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.2 overexpression in Escherichia coli Citrobacter amalonaticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.2
-
 Citrobacter amalonaticus
4.3.1.3 structure solved to 1.8 A resolution Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.2 0.13
-
 mesaconic acid
-
 Citrobacter amalonaticus
4.3.1.2 0.76
-
 (2S,3S)-3-methylaspartate
-
 Citrobacter amalonaticus
4.3.1.2 7.9
-
 (2R,3S)-3-chloroaspartic acid
-
 Citrobacter amalonaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.2 Co2+ divalent cation requirement, cations in order of decreasing efficiency: Mg2+, Mn2+, Co2+ Citrobacter amalonaticus
4.3.1.2 Cs+ monovalent cation requirement, cations in decreasing order of efficiency: K+, Mn2+, Cs+ Citrobacter amalonaticus
4.3.1.2 K+ monovalent cation requirement, cations in decreasing order of efficiency: K+, Mn2+, Cs+ Citrobacter amalonaticus
4.3.1.2 Mg2+ divalent cation requirement, cations in order of decreasing efficiency: Mg2+, Mn2+, Co2+ Citrobacter amalonaticus
4.3.1.2 Mn2+ divalent cation requirement, cations in order of decreasing efficiency: Mg2+, Mn2+, Co2+ Citrobacter amalonaticus
4.3.1.2 Rb+ monovalent cation requirement, cations in decreasing order of efficiency: K+, Mn2+, Cs+ Citrobacter amalonaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 40000
-
 2 * 40000 Citrobacter freundii
4.3.1.2 42000
-
 2 * 42000 Citrobacter amalonaticus
4.3.1.2 44000
-
 2 * 44000 Morganella morganii
4.3.1.2 70000
-
 gel filtration Morganella morganii
4.3.1.2 79000
-
 gel filtration Citrobacter freundii
4.3.1.2 84000
-
 gel filtration Citrobacter amalonaticus

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Citrobacter amalonaticus
-
 YG-1002
-
4.3.1.2 Citrobacter amalonaticus YG-1002
-
 YG-1002
-
4.3.1.2 Citrobacter freundii
-
 YG.0504
-
4.3.1.2 Citrobacter freundii YG.0504
-
 YG.0504
-
4.3.1.2 Clostridium tetanomorphum
-
-
-
4.3.1.2 Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
4.3.1.2 Morganella morganii
-
 YG-0601
-
4.3.1.2 Morganella morganii YG-0601
-
 YG-0601
-
4.3.1.3 Pseudomonas putida
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.3.1.3 additional information the peptide at the active center of the enzyme is considered to be posttranslationally dehydrated to form an electrophilic 4-methylidene-imidazole-one group Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 (2R,3S)-3-chloroaspartic acid
-
 Citrobacter amalonaticus 2-chloro-2-butenedioic acid
-
 ?
4.3.1.2 (2R,3S)-3-chloroaspartic acid
-
 Citrobacter amalonaticus YG-1002 2-chloro-2-butenedioic acid
-
 ?
4.3.1.2 (2S,3S)-3-methylaspartate
-
 Citrobacter amalonaticus mesaconate + NH3
-
 r
4.3.1.2 (2S,3S)-3-methylaspartate
-
 Citrobacter amalonaticus YG-1002 mesaconate + NH3
-
 r
4.3.1.2 additional information no reaction with aspartic acid Citrobacter amalonaticus ?
-
 ?
4.3.1.2 additional information no reaction with aspartic acid Citrobacter amalonaticus YG-1002 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.2 dimer 2 * 42000 Citrobacter amalonaticus
4.3.1.2 dimer 2 * 40000 Citrobacter freundii
4.3.1.2 dimer 2 * 44000 Morganella morganii
4.3.1.3 tetramer
-
 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.3.1.2 3-methylaspartate ammonia-lyase
-
 Citrobacter freundii
4.3.1.2 3-methylaspartate ammonia-lyase
-
 Clostridium tetanomorphum
4.3.1.2 3-methylaspartate ammonia-lyase
-
 Morganella morganii
4.3.1.2 3-methylaspartate ammonia-lyase
-
 Citrobacter amalonaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.2 45 50
-
 Citrobacter amalonaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.2 8 8.5 amination Citrobacter amalonaticus
4.3.1.2 9.7
-
 deamination Citrobacter amalonaticus