EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.76 | 2-mercaptoethanol | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | 4-hydroxymercuribenzoate | - |
Pseudomonas indica | |
3.1.1.76 | 4-hydroxymercuribenzoate | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | diisopropylfluorophosphate | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | dithiothreitol | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | DTNB | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | EDTA | - |
Pseudomonas indica | |
3.1.1.76 | EDTA | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | ethanol | strong inhibition at 10% v/v | Schlegelella thermodepolymerans | |
3.1.1.76 | iodoacetate | - |
Pseudomonas indica | |
3.1.1.76 | iodoacetate | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | KCl | - |
Schlegelella thermodepolymerans | |
3.1.1.76 | additional information | no inhibition by DTT | Pseudomonas indica | |
3.1.1.76 | n-Propanol | strong inhibition at 10% v/v | Schlegelella thermodepolymerans | |
3.1.1.76 | NaCl | - |
Schlegelella thermodepolymerans | |
3.1.1.76 | PMSF | 40% inhibition 1.0-10 mM | Schlegelella thermodepolymerans | |
3.1.1.76 | Sodium azide | - |
Pseudomonas indica | |
3.1.1.76 | Sodium azide | complete inhibition | Schlegelella thermodepolymerans | |
3.1.1.76 | Triton X-100 | strong inhibition | Pseudomonas indica | |
3.1.1.76 | Triton X-100 | strong inhibition 0.2-1.0 mM | Schlegelella thermodepolymerans | |
3.1.1.76 | Tween 20 | 66% inhibition 2 mM | Schlegelella thermodepolymerans | |
3.1.1.76 | Tween 80 | strong inhibition | Pseudomonas indica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.76 | extracellular | - |
Schlegelella thermodepolymerans | - |
- |
3.1.1.76 | extracellular | - |
Pseudomonas indica | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 28000 | - |
x * 28000, SDS-PAGE | Schlegelella thermodepolymerans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.76 | Pseudomonas indica | - |
- |
- |
3.1.1.76 | Pseudomonas indica K2 / DSM 16298 | - |
- |
- |
3.1.1.76 | Schlegelella thermodepolymerans | - |
strain DSM 15344 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.76 | to homogeneity by ultrafiltration, affinity-based separation, and gel filtration | Pseudomonas indica |
3.1.1.76 | to homogeneity by ultrafiltration, heat tretament at 70°C for 30 min, and affinity-based separation | Schlegelella thermodepolymerans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.76 | additional information | the bacterium can grow on 3-mercaptopropionate or poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source | Schlegelella thermodepolymerans | - |
3.1.1.76 | additional information | the bacterium can grow on poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source | Pseudomonas indica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 25 | - |
purified enzyme | Schlegelella thermodepolymerans |
3.1.1.76 | 26 | - |
purified enzyme | Pseudomonas indica |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.76 | -20°C to 70°C, purified enzyme, stable for more than 6 months | Pseudomonas indica |
3.1.1.76 | -20°C, purified enzyme, 1 year, 70% remaining activity | Schlegelella thermodepolymerans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.76 | additional information | substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate | Schlegelella thermodepolymerans | ? | - |
? | |
3.1.1.76 | additional information | substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) | Pseudomonas indica | ? | - |
? | |
3.1.1.76 | additional information | substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) | Pseudomonas indica K2 / DSM 16298 | ? | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-mercaptopropionate) + H2O | co-polymeric substrate, the activity decreases with increasing amount of 3-mercaptopropionate | Schlegelella thermodepolymerans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.76 | ? | x * 28000, SDS-PAGE | Schlegelella thermodepolymerans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.76 | PHA depolymerase | - |
Schlegelella thermodepolymerans |
3.1.1.76 | PHA depolymerase | - |
Pseudomonas indica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | additional information | - |
optimal bacterial growth at 30°C | Pseudomonas indica |
3.1.1.76 | additional information | - |
optimal bacterial growth at 55°C | Schlegelella thermodepolymerans |
3.1.1.76 | 35 | - |
- |
Pseudomonas indica |
3.1.1.76 | 75 | 90 | - |
Schlegelella thermodepolymerans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 15 | 50 | - |
Pseudomonas indica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 50 | - |
over 90% remaining activity after 24 h | Schlegelella thermodepolymerans |
3.1.1.76 | 70 | - |
69% remaining activity after 24 h | Schlegelella thermodepolymerans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 8 | - |
assay at | Schlegelella thermodepolymerans |
3.1.1.76 | 8 | - |
assay at | Pseudomonas indica |