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Literature summary extracted from

  • Naseri, J.I.; Truong, N.T.; Horentrup, J.; Kuballa, P.; Vogel, A.; Rompel, A.; Spener, F.; Krebs, B.
    Porcine purple acid phosphatase: heterologous expression, characterization, and proteolytic analysis (2004), Arch. Biochem. Biophys., 432, 25-36.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 Trypsin partial cleavage results in 1.7fold enzyme activation Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expression in Spodopetra frugiperda Sf9 cells using the baculovirus infection system, the recombinant enzyme is secreted from the cell culture Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Pepsin partial cleavage results in enzyme inhibition Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe2+ two iron ions per enzyme molecule Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 67000
-
1 * 35000-37000 + 1 * 67000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.2 additional information Sus scrofa the enzyme is an iron-binding protein required for maternal/fetal iron transport ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein recombinant enzyme Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 recombinant secreted enzyme 155fold from Sf9 insect cell culture expressing the enzyme by ion exchange, adsorption, and affinity chromatography, and gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 uterine endometrium
-
Sus scrofa
-
3.1.3.2 uterus
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 1.5
-
recombinant enzyme in cell culture medium Sus scrofa
3.1.3.2 233
-
purified recombinant enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
Sus scrofa 4-nitrophenol + phosphate
-
?
3.1.3.2 additional information the enzyme is an iron-binding protein required for maternal/fetal iron transport Sus scrofa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 1 * 35000-37000 + 1 * 67000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 PAP
-
Sus scrofa
3.1.3.2 purple acid phosphatase
-
Sus scrofa
3.1.3.2 Uf
-
Sus scrofa
3.1.3.2 uteroferrin
-
Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
assay at Sus scrofa