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Literature summary extracted from

  • Jong, A.; Yeh, Y.; Ma, J.J.
    Characteristics, substrate analysis, and intracellular location of Saccharomyces cerevisiae UMP kinase (1993), Arch. Biochem. Biophys., 304, 197-204.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.B1 5,5'-dithio-bis(2-nitrobenzoic acid)
-
 Saccharomyces cerevisiae
2.7.4.B1 N-ethylmaleimide
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.B1 cytoplasm 80% Saccharomyces cerevisiae 5737
-
2.7.4.B1 nucleus 20% Saccharomyces cerevisiae 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.B1 Ca2+ 57% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Cd2+ no activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Co2+ 99% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Cu2+ 11% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Fe2+ 24% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Mg2+ 100% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Mn2+ 100% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Ni2+ 84% activity at 7.5 mM Saccharomyces cerevisiae
2.7.4.B1 Zn2+ 14% activity at 7.5 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.B1 25000
-
 monomer, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.B1 ATP + UMP Saccharomyces cerevisiae
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.B1 Saccharomyces cerevisiae P15700
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.B1 to homogeneity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.B1 ATP + 5-iodo-dUMP reaction occurs to a lesser extent Saccharomyces cerevisiae ADP + 5-iodo-dUDP
-
 ?
2.7.4.B1 ATP + dGMP reaction occurs to a lesser extent Saccharomyces cerevisiae ADP + dGDP
-
 ?
2.7.4.B1 ATP + dTMP reaction occurs to a lesser extent Saccharomyces cerevisiae ADP + dTDP
-
 ?
2.7.4.B1 ATP + dUMP
-
 Saccharomyces cerevisiae ADP + dUDP
-
 ?
2.7.4.B1 ATP + GMP reaction occurs to a lesser extent Saccharomyces cerevisiae ADP + GDP
-
 ?
2.7.4.B1 ATP + IMP reaction occurs to a lesser extent Saccharomyces cerevisiae ADP + IDP
-
 ?
2.7.4.B1 ATP + UMP
-
 Saccharomyces cerevisiae ADP + UDP
-
 ?
2.7.4.B1 ATP + UMP pH 7.5, 7.5 mM Mg2+, 30°C Saccharomyces cerevisiae ADP + UDP
-
 ?
2.7.4.B1 ATP + XMP reaction occurs to a lesser extent Saccharomyces cerevisiae ADP + XDP
-
 ?
2.7.4.B1 CTP + UMP reaction rate is 10-14% of that with ATP or dATP Saccharomyces cerevisiae CDP + UDP
-
 ?
2.7.4.B1 dATP + UMP
-
 Saccharomyces cerevisiae dADP + UDP
-
 ?
2.7.4.B1 dCTP + UMP reaction rate is 30-50% of that with ATP or dATP Saccharomyces cerevisiae dCDP + UDP
-
 ?
2.7.4.B1 dGTP + UMP reaction rate is 30-50% of that with ATP or dATP Saccharomyces cerevisiae dGDP + UDP
-
 ?
2.7.4.B1 dTTP + UMP reaction rate is 30-50% of that with ATP or dATP Saccharomyces cerevisiae dTDP + UDP
-
 ?
2.7.4.B1 GTP + UMP reaction rate is 30-50% of that with ATP or dATP Saccharomyces cerevisiae GDP + UDP
-
 ?
2.7.4.B1 UTP + UMP reaction rate is 10-14% of that with ATP or dATP Saccharomyces cerevisiae UDP + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.B1 UMP kinase
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.B1 50
-
 enzyme activity decreases to 55%. In the presence of 1 mM UTP enzyme activity decreases to 73%. In the presence of 10% glycerol enzyme activity decreases to 77%. In the presence of 10% polyethylene glycol enzyme activity decreases to 95%. In the presence of 10 mM DTT enzyme activity decreases to 80% Saccharomyces cerevisiae
2.7.4.B1 60
-
 enzyme activity decreases to 3%. In the presence of 1 mM UTP enzyme activity decreases to 10%. In the presence of 10% glycerol enzyme activity decreases to 14%. In the presence of 10% polyethylene glycol enzyme activity decreases to 34%. In the presence of 10 mM DTT enzyme activity decreases to 68% Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.B1 6 9.5
-
 Saccharomyces cerevisiae