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Literature summary extracted from

  • Herter, T.; Berezina, O.V.; Zinin, N.V.; Velikodvorskaya, G.A.; Greiner, R.; Borriss, R.
    Glucose-1-phosphatase (AgpE) from Enterobacter cloacae displays enhanced phytase activity (2006), Appl. Microbiol. Biotechnol., 70, 60-64.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.10
-
 Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.10 0.31
-
 D-glucose 1-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 0.47
-
 D-glucose 6-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 0.51
-
 myo-inositol hexakisphosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 0.62
-
 D-Fructose 1-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 0.66
-
 D-fructose 6-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 13
-
 p-nitrophenyl phosphate 37°C, pH 5.0 Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.10 44000
-
 2 * 44000, SDS-PAGE Enterobacter cloacae
3.1.3.10 82500
-
 gel filtration Enterobacter cloacae
3.1.3.10 125000
-
 PAGE Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.10 Enterobacter cloacae
-
 enzyme additionally can act as 3-phytase but is only able to cleave off the third phosphate group of myo-inositol
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.10 32
-
 substrate myo-inositol hexakisphosphate, 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 40
-
 substrate D-fructose 6-phosphate, 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 142
-
 substrate D-fructose 1-phosphate, 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 146
-
 substrate D-glucose 1-phosphate, 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 159
-
 substrate D-glucose 6-phosphate, 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 196
-
 substrate D-glucose 1-phosphate, 37°C, pH 5.0 Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.10 D-fructose 1-phosphate + H2O
-
 Enterobacter cloacae D-fructose + phosphate
-
 ?
3.1.3.10 D-fructose 6-phosphate + H2O
-
 Enterobacter cloacae D-fructose + phosphate
-
 ?
3.1.3.10 D-glucose 1-phosphate + H2O
-
 Enterobacter cloacae D-glucose + phosphate
-
 ?
3.1.3.10 D-glucose 6-phosphate + H2O
-
 Enterobacter cloacae D-glucose + phosphate
-
 ?
3.1.3.10 myo-inositol hexakisphosphate + H2O
-
 Enterobacter cloacae ? + phosphate enzyme is only able to cleave off the third phosphate group of myo-inositol ?
3.1.3.10 p-nitrophenyl phosphate + H2O
-
 Enterobacter cloacae p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.10 dimer 2 * 44000, SDS-PAGE Enterobacter cloacae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.10 AgpE
-
 Enterobacter cloacae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.10 24
-
 myo-inositol hexakisphosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 30
-
 p-nitrophenyl phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 105
-
 D-fructose 6-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 109
-
 D-glucose 6-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 111
-
 D-Fructose 1-phosphate 37°C, pH 5.0 Enterobacter cloacae
3.1.3.10 145
-
 D-glucose 1-phosphate 37°C, pH 5.0 Enterobacter cloacae