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Literature summary extracted from
- Sedolisins, a new class of secreted proteases from Aspergillus fumigatus with endoprotease or tripeptidyl-peptidase activity at acidic pHs (2006), Appl. Environ. Microbiol., 72, 1739-1748.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.100 | medicine | potential importance as a virulence factor and in the industrial hydrolysis of protein source | Aspergillus fumigatus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.14.9 | expression of SedB in Pichia pastoris | Aspergillus fumigatus |
| 3.4.14.9 | expression of SedC in Pichia pastoris | Aspergillus fumigatus |
| 3.4.14.9 | expression of SedD in Pichia pastoris | Aspergillus fumigatus |
| 3.4.21.100 | SedA and SedC cloned into vector pUC18. Heterologously expressed in Pichia pastoris. SedA, SedB, SedC and SedD cloned into vector pET-11aH6 and expressed in Escherichia coli BL21 | Aspergillus fumigatus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.21.100 | additional information | sedB-, sedC-, and sedD-negative mutants have 20%, 90%, and 80% of the tripeptidyl-peptidase activity of wild-type. None of the sed-negative mutants have a visible phenotype on Sabouraud agar or in hemoglobin liquid medium | Aspergillus fumigatus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.9 | 0.0625 | - |
Phe-Pro-Ala-4-nitroanilide | pH 6.0, 20°C, SedB | Aspergillus fumigatus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 40600 | - |
x * 40600, SedD, calculated from sequence | Aspergillus fumigatus |
| 3.4.14.9 | 43000 | - |
x * 43000, SedB, calculated from sequence | Aspergillus fumigatus |
| 3.4.21.100 | 46000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 3.4.21.100 | 48000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 3.4.21.100 | 55000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 3.4.21.100 | 65000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 3.4.21.100 | 406000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 3.4.21.100 | 430000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 3.4.21.100 | 479000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.9 | Aspergillus fumigatus | Q70GH4 | FuSed3 protease precursor | - |
| 3.4.14.9 | Aspergillus fumigatus | Q70J58 | FuSed4 protease precursor | - |
| 3.4.14.9 | Aspergillus fumigatus | Q70J59 | FuSed2 protease precursor | - |
| 3.4.21.100 | Aspergillus fumigatus | - |
- |
- |
| 3.4.21.100 | Aspergillus fumigatus NCPF 7367 | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.14.9 | proteolytic modification | the preproprotein SedB is composed of 602 amino acids, the signal peptide contains 24 amino acids | Aspergillus fumigatus |
| 3.4.14.9 | proteolytic modification | the preproprotein SedC is composed of 596 amino acids, the signal peptide contains 24 amino acids | Aspergillus fumigatus |
| 3.4.14.9 | proteolytic modification | the preproprotein SedD is composed of 568 amino acids, the signal peptide contains 20 amino acids | Aspergillus fumigatus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.9 | recombinant SedB | Aspergillus fumigatus |
| 3.4.14.9 | recombinant SedD | Aspergillus fumigatus |
| 3.4.21.100 | SedB purified by ion-exchange chromatography and gel filtration | Aspergillus fumigatus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.100 | additional information | SedB, SedC, and SedD in culture supernatants grown in a medium containing hemoglobin as the sole nitrogen source | Aspergillus fumigatus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.9 | Ala-Ala-Phe-4-nitroanilide + H2O | SedB | Aspergillus fumigatus | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Ala-Ala-Phe-4-nitroanilide + H2O | SedC | Aspergillus fumigatus | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Ala-Ala-Phe-4-nitroanilide + H2O | SedD | Aspergillus fumigatus | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O | SedB | Aspergillus fumigatus | Ala-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe | - |
? | |
| 3.4.14.9 | Phe-Pro-Ala-4-nitroanilide + H2O | SedB | Aspergillus fumigatus | Phe-Pro-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Phe-Pro-Ala-4-nitroanilide + H2O | SedC | Aspergillus fumigatus | Phe-Pro-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Phe-Pro-Ala-4-nitroanilide + H2O | SedD | Aspergillus fumigatus | Phe-Pro-Ala + 4-nitroaniline | - |
? | |
| 3.4.21.100 | Ala-Ala-Phe-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus | Ala-Ala-Phe + p-nitroaniline | - |
? | |
| 3.4.21.100 | Ala-Ala-Phe-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus NCPF 7367 | Ala-Ala-Phe + p-nitroaniline | - |
? | |
| 3.4.21.100 | Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O | SedB | Aspergillus fumigatus | Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe | - |
? | |
| 3.4.21.100 | Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O | SedB | Aspergillus fumigatus NCPF 7367 | Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe | - |
? | |
| 3.4.21.100 | additional information | SedA is an acidic endoprotease. SedB, SedC, and SedD are tripeptidyl-peptidases with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs | Aspergillus fumigatus | ? | - |
? | |
| 3.4.21.100 | additional information | SedA is an acidic endoprotease. SedB, SedC, and SedD are tripeptidyl-peptidases with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs | Aspergillus fumigatus NCPF 7367 | ? | - |
? | |
| 3.4.21.100 | Phe-Pro-Ala-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus | Phe-Pro-Ala + p-nitroaniline | - |
? | |
| 3.4.21.100 | Phe-Pro-Ala-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus NCPF 7367 | Phe-Pro-Ala + p-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.9 | ? | x * 40600, SedD, calculated from sequence | Aspergillus fumigatus |
| 3.4.14.9 | ? | x * 43000, SedB, calculated from sequence | Aspergillus fumigatus |
| 3.4.21.100 | ? | x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.14.9 | SedB | - |
Aspergillus fumigatus |
| 3.4.14.9 | SedC | - |
Aspergillus fumigatus |
| 3.4.14.9 | sedolisin B | - |
Aspergillus fumigatus |
| 3.4.14.9 | sedolisin C | - |
Aspergillus fumigatus |
| 3.4.14.9 | sedolisin D | - |
Aspergillus fumigatus |
| 3.4.21.100 | SedA | - |
Aspergillus fumigatus |
| 3.4.21.100 | SedB | - |
Aspergillus fumigatus |
| 3.4.21.100 | SedC | - |
Aspergillus fumigatus |
| 3.4.21.100 | SedD | - |
Aspergillus fumigatus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.9 | 35 | - |
Phe-Pro-Ala-4-nitroanilide | pH 6.0, 20°C, SedB | Aspergillus fumigatus | |
| 3.4.21.100 | 35 | - |
Phe-Pro-Ala-p-nitroanilide | - |
Aspergillus fumigatus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 5 | - |
SedD | Aspergillus fumigatus |
| 3.4.14.9 | 6 | - |
SedB | Aspergillus fumigatus |
| 3.4.14.9 | 6 | - |
SedC | Aspergillus fumigatus |
| 3.4.21.100 | additional information | - |
SedB and SedC have an optimum at pH 6.0, while SedD has an optimum peak at pH 5.0 | Aspergillus fumigatus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 1.5 | 6 | active between pH 3 and pH 7, SedD | Aspergillus fumigatus |
| 3.4.14.9 | 3 | 7 | active between pH 3 and pH 7, SedB | Aspergillus fumigatus |
| 3.4.14.9 | 3 | 7 | active between pH 3 and pH 7, SedC | Aspergillus fumigatus |
| 3.4.21.100 | 1.5 | 7 | SedB and SedC are active between pH 3.0 and 7.0, while SedD is active between pH 1.5 and 6.0 | Aspergillus fumigatus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.14.9 | Aspergillus fumigatus | calculated from sequence, mature domain, SedD | - |
5.12 |
| 3.4.14.9 | Aspergillus fumigatus | calculated from sequence, mature domain, SedB | - |
5.17 |
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