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Literature summary extracted from

  • Wang, F.; Cassidy, C.; Sacchettini, J.C.
    Crystal structure and activity studies of the Mycobacterium tuberculosis beta-lactamase reveal its critical role in resistance to beta-lactam antibiotics (2006), Antimicrob. Agents Chemother., 50, 2762-2771.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli Mycobacterium tuberculosis
3.5.2.6 plasmid pET28b is transformed into Escherichia coli BL21(DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 hanging drop vapour diffusion method with 0.1 M Tris-HCl, pH 8.0, 2.0 M NH4H2PO4 Mycobacterium tuberculosis
3.5.2.6 hanging-drop vapour-diffusion method, 1.2 A resolution Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 additional information the amino acid substitutions N132G, R164A, R244A, and R276E explain the broad specificity of the enzyme, relatively low penicillinase activity, and resistance to clavulanic acid Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanate
-
 Mycobacterium tuberculosis
3.5.2.6 clavulanic acid poor inhibitor Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.063
-
 ampicillin
-
 Mycobacterium tuberculosis
3.5.2.6 0.063
-
 ampicillin at 30°C Mycobacterium tuberculosis
3.5.2.6 0.117
-
 cephalothin
-
 Mycobacterium tuberculosis
3.5.2.6 0.117
-
 cephalothin at 30°C Mycobacterium tuberculosis
3.5.2.6 0.195
-
 cefoxitin
-
 Mycobacterium tuberculosis
3.5.2.6 0.195
-
 cefoxitin at 30°C Mycobacterium tuberculosis
3.5.2.6 0.279
-
 meropenem
-
 Mycobacterium tuberculosis
3.5.2.6 0.279
-
 meropenem at 30°C Mycobacterium tuberculosis
3.5.2.6 0.593
-
 ceftazidime
-
 Mycobacterium tuberculosis
3.5.2.6 0.593
-
 ceftazidime at 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 30630
-
 truncated protein, matrix-assisted laser desorption ionization Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Mycobacterium tuberculosis
3.5.2.6 Ni2+-loaded HiTrap chelating column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Mycobacterium tuberculosis (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O low activity Mycobacterium tuberculosis (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Mycobacterium tuberculosis (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Mycobacterium tuberculosis (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Mycobacterium tuberculosis (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Mycobacterium tuberculosis (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 BlaC
-
 Mycobacterium tuberculosis
3.5.2.6 class A beta-lactamase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.2
-
 ceftazidime
-
 Mycobacterium tuberculosis
3.5.2.6 0.2
-
 ceftazidime at 30°C Mycobacterium tuberculosis
3.5.2.6 0.9
-
 meropenem
-
 Mycobacterium tuberculosis
3.5.2.6 0.9
-
 meropenem at 30°C Mycobacterium tuberculosis
3.5.2.6 1.1
-
 cefoxitin
-
 Mycobacterium tuberculosis
3.5.2.6 1.1
-
 cefoxitin at 30°C Mycobacterium tuberculosis
3.5.2.6 12.1
-
 cephalothin
-
 Mycobacterium tuberculosis
3.5.2.6 12.1
-
 cephalothin at 30°C Mycobacterium tuberculosis
3.5.2.6 18.5
-
 ampicillin
-
 Mycobacterium tuberculosis
3.5.2.6 18.5
-
 ampicillin at 30°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.0024
-
 clavulanic acid
-
 Mycobacterium tuberculosis
3.5.2.6 0.0024
-
 clavulanate
-
 Mycobacterium tuberculosis