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Literature summary extracted from

  • Leonard, P.M.; Marshall, C.M.; Dodson, E.J.; Walton, N.J.; Grogan, G.
    Purification, crystallization and preliminary x-ray crystallographic analysis of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL), a crotonase homologue active in phenylpropanoid metabolism (2004), Acta Crystallogr. Sect. D, D60, 2343-2345.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.61 expression in Escherichia coli strain BL21(DE3) Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.61 by the hanging-drop method of vapour diffusion using polyethylene glycol 20000 Da as the precipitant, 1.8 A resolution Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.61 31007
-
 6 * 31007, crystallization, calculated from amino acid sequence Pseudomonas fluorescens
4.1.2.61 186000
-
 calculated from crystallization data Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens AN103
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.61 Pseudomonas fluorescens
-
-
-
4.1.2.61 Pseudomonas fluorescens AN103
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.61 recombinant protein from Escherichia coli Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens AN103 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.61 hexamer 6 * 31007, crystallization, calculated from amino acid sequence Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 4-hydroxycinnamoyl-coenzyme A hydratase-lyase
-
 Pseudomonas fluorescens
4.1.2.61 FCHL
-
 Pseudomonas fluorescens
4.1.2.61 feruloyl-CoA hydratase-lyase
-
 Pseudomonas fluorescens
4.1.2.61 HCHL
-
 Pseudomonas fluorescens