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Literature summary extracted from

  • Tkacz-Stachowska, K.; Lechward, K.; Skladanowski, A.C.
    Isolation and characterization of pigeon breast muscle cytosolic 5'-nucleotidase-I (cN-I) (2005), Acta Biochim. Pol., 52, 789-796.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 ADP strong, allosteric activiator Columba livia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 phosphate
-
 Columba livia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.04
-
 5'-dCMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
3.1.3.5 0.29
-
 5'-AMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
3.1.3.5 0.75
-
 5'-dCMP 37°C, pH 7.0 Columba livia
3.1.3.5 1.67
-
 5'-IMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
3.1.3.5 1.85
-
 5'-AMP 37°C, pH 7.0 Columba livia
3.1.3.5 2 3 5'-dAMP 37°C, pH 7.0 Columba livia
3.1.3.5 5.2
-
 5'-dAMP 37°C, pH 7.0, presence of 1 mM ADP Columba livia
3.1.3.5 10.8
-
 5'-IMP 37°C, pH 7.0 Columba livia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytosol
-
 Columba livia 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 43000
-
 x * 43000, SDS-PAGE Columba livia

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Columba livia
-
 isoform cN-I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Columba livia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 muscle breast muscle Columba livia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 4.33
-
 37°C, pH 7.0 Columba livia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O absence of ADP, 60% loss of activity Columba livia adenosine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O presence of ADP, 66% of the activity with 5’-AMP Columba livia deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O presence of ADP, 16% of the activity with 5’-AMP Columba livia deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O presence of ADP, 38% of the activity with 5’-AMP Columba livia deoxyinosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 43000, SDS-PAGE Columba livia