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Literature summary extracted from

  • Operario, D.J.; Reynolds, H.M.; Kim, B.
    Comparison of DNA polymerase activities between recombinant feline immunodeficiency and leukemia virus reverse transcriptases (2005), Virology, 335, 106-121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.49
-
 feline leukemia virus
2.7.7.49
-
 feline immunodeficiency virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.49 3'-azido-3'deoxythymidine 5'-triphosphate IC50: 0.042 mM feline immunodeficiency virus
2.7.7.49 3'-azido-3'deoxythymidine 5'-triphosphate IC50: 0.06 mM feline leukemia virus
2.7.7.49 ddATP 0.015 mM feline immunodeficiency virus
2.7.7.49 ddATP 0.28 mM feline leukemia virus
2.7.7.49 lamivudine triphosphate
-
 feline immunodeficiency virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.49 0.000028
-
 dCTP
-
 feline immunodeficiency virus
2.7.7.49 0.000051
-
 dTTP
-
 feline immunodeficiency virus
2.7.7.49 0.00006
-
 dGTP
-
 feline immunodeficiency virus
2.7.7.49 0.000081
-
 dATP
-
 feline immunodeficiency virus
2.7.7.49 0.000936
-
 dGTP
-
 feline leukemia virus
2.7.7.49 0.00148
-
 dCTP
-
 feline leukemia virus
2.7.7.49 0.00255
-
 dTTP
-
 feline leukemia virus
2.7.7.49 0.004674
-
 dATP
-
 feline leukemia virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.49 Mg2+ 5 mM, enzyme shows high activity in presence of feline immunodeficiency virus
2.7.7.49 Mn2+ enzyme can effectively use Mn2+ at 0.05 mM feline immunodeficiency virus
2.7.7.49 Mn2+ enzyme can effectively use Mn2+ at 0.05 mM, enzyme does not efficiently use Mg2+ feline leukemia virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.49 feline immunodeficiency virus
-
-
-
2.7.7.49 feline leukemia virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.49
-
 feline leukemia virus
2.7.7.49
-
 feline immunodeficiency virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.49 dATP + DNAn
-
 feline leukemia virus diphosphate + DNAn+1
-
 ?
2.7.7.49 dATP + DNAn
-
 feline immunodeficiency virus diphosphate + DNAn+1
-
 ?
2.7.7.49 dCTP + DNAn
-
 feline leukemia virus diphosphate + DNAn+1
-
 ?
2.7.7.49 dCTP + DNAn
-
 feline immunodeficiency virus diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
 feline leukemia virus diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
 feline immunodeficiency virus diphosphate + DNAn+1
-
 ?
2.7.7.49 dGTP + DNAn
-
 feline leukemia virus diphosphate + DNAn+1
-
 ?
2.7.7.49 dGTP + DNAn
-
 feline immunodeficiency virus diphosphate + DNAn+1
-
 ?
2.7.7.49 dTTP + poly(rA)/(dT)18
-
 feline leukemia virus ?
-
 ?
2.7.7.49 dTTP + poly(rA)/(dT)18
-
 feline immunodeficiency virus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.49 FeLV RT
-
 feline leukemia virus
2.7.7.49 FIV RT
-
 feline immunodeficiency virus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.49 0.15
-
 dCTP
-
 feline leukemia virus
2.7.7.49 0.17
-
 dTTP
-
 feline leukemia virus
2.7.7.49 0.18
-
 dATP
-
 feline leukemia virus
2.7.7.49 0.21
-
 dATP
-
 feline immunodeficiency virus
2.7.7.49 0.22
-
 dCTP
-
 feline immunodeficiency virus
2.7.7.49 0.28
-
 dTTP
-
 feline immunodeficiency virus
2.7.7.49 0.51
-
 dGTP
-
 feline immunodeficiency virus
2.7.7.49 0.56
-
 dGTP
-
 feline leukemia virus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.49 0.042
-
 IC50: 0.042 mM feline immunodeficiency virus 3'-azido-3'deoxythymidine 5'-triphosphate
2.7.7.49 0.06
-
 IC50: 0.06 mM feline leukemia virus 3'-azido-3'deoxythymidine 5'-triphosphate