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Literature summary extracted from

  • Gardberg, A.; Shuvalova, L.; Monnerjahn, C.; Konrad, M.; Lavie, A.
    Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases (2003), Structure, 11, 1265-1277.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expression of the His-tagged, thrombin-cleavable full length or truncated enzyme in Escherichia coli strain BL21 C41 (DE3) Herpes simplex virus type 4
2.7.4.9 full length and truncated enzyme lacking the first 22 amino acids expressed in Escherichia coli BL21 C41 (DE3) Equid alphaherpesvirus 4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.21 full length and truncated enzyme in complex with thymidine and ADP, or with thymidine, sulfate, and a bisubstrate analogues, or with TP4A, or with TP5A, hanging drop vapour diffusion method, reservoir solution containing 0.1 M MES, pH 6.5, 1.2 M ammonium sulfate, and 3 or 5% dioxane, dependent on the complex ligand, mixed with enzyme solution containing 5.5 mg/ml protein, 1.0 mM TMP, 2.0 mM ADP, 25 mM HEPES, pH 7.0, 5 mM MgSO4, 5 mM DTT, 100 mM KCl, and 5% glycerol, at equal volumes of 0.001 ml on a glass cover slide, X-ray diffraction structure determination and analysis at 2.0-3.0 A resolution, molecular replacement method Herpes simplex virus type 4
2.7.4.9 hanging drop vapor diffusion method Equid alphaherpesvirus 4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 36500
-
x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation Herpes simplex virus type 4
2.7.1.21 38800
-
x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation Herpes simplex virus type 4
2.7.4.9 36500
-
2 * 36500, calculated from the deduced amino acid sequence, crystal structure analysis Equid alphaherpesvirus 4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + thymidine Herpes simplex virus type 4
-
ADP + thymidine 5'-phosphate
-
r
2.7.1.21 ATP + thymidine 5'-phosphate Herpes simplex virus type 4
-
ADP + thymidine diphosphate
-
r
2.7.4.9 ATP + thymidine Herpes simplex virus
-
ADP + TMP
-
?
2.7.4.9 ATP + thymidine Equid alphaherpesvirus 4
-
ADP + TMP
-
?
2.7.4.9 ATP + TMP Herpes simplex virus
-
ADP + TDP
-
?
2.7.4.9 ATP + TMP Equid alphaherpesvirus 4
-
ADP + TDP
-
?
2.7.4.9 additional information Herpes simplex virus multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues ?
-
?
2.7.4.9 additional information Equid alphaherpesvirus 4 multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Herpes simplex virus type 4
-
type 4
-
2.7.4.9 Equid alphaherpesvirus 4
-
virus type 4
-
2.7.4.9 Herpes simplex virus
-
virus type 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 recombinant His-tagged, thrombin-cleavable full length or truncated enzyme from Escherichia coli strain BL21 C41 (DE3) by nickel affinity chromatography, cleavage by thrombin Herpes simplex virus type 4
2.7.4.9 truncated recombinant enzyme, full length enzyme could not be crystallized Equid alphaherpesvirus 4

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.21 ATP + thymidine = ADP + dTMP substrate binding site structures, structural basis for substrate specificity and activity Herpes simplex virus type 4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine
-
Herpes simplex virus type 4 ADP + thymidine 5'-phosphate
-
r
2.7.1.21 ATP + thymidine 5'-phosphate
-
Herpes simplex virus type 4 ADP + thymidine diphosphate
-
r
2.7.1.21 additional information wide substrate specificity phosphorylating pyrimidine nucleosides, nucleoside monophophates, and several nucleoside analogues Herpes simplex virus type 4 ?
-
?
2.7.4.9 ATP + thymidine
-
Herpes simplex virus ADP + TMP
-
?
2.7.4.9 ATP + thymidine
-
Equid alphaherpesvirus 4 ADP + TMP
-
?
2.7.4.9 ATP + TMP
-
Herpes simplex virus ADP + TDP
-
?
2.7.4.9 ATP + TMP
-
Equid alphaherpesvirus 4 ADP + TDP
-
?
2.7.4.9 additional information multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues Herpes simplex virus ?
-
?
2.7.4.9 additional information multifunctional enzyme which also phosphorylates pyrimidine nucleosides, pyrimidine nucleoside monophosphates, and several nucleoside analogues Equid alphaherpesvirus 4 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 ? x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation Herpes simplex virus type 4
2.7.4.9 dimer 2 * 36500, calculated from the deduced amino acid sequence, crystal structure analysis Equid alphaherpesvirus 4

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 EHV4-TK
-
Herpes simplex virus type 4
2.7.1.21 thymidylate kinase
-
Herpes simplex virus type 4

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 ATP
-
Herpes simplex virus type 4