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Literature summary extracted from

  • Kothe, M.; Purcarea, C.; Guy, H.I.; Evans, D.R.; Powers-Lee, S.G.
    Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase (2005), Protein Sci., 14, 37-44.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.5 subcloning of each of the subunits into the pET-28b vector, expression in Escherichia coli Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 56000
-
 x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE Methanocaldococcus jannaschii
6.3.5.5 71000
-
 x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- cross-talk between domain N and C is required for product release from subunit C Methanocaldococcus jannaschii 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 ? x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE Methanocaldococcus jannaschii