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Literature summary extracted from

  • Hsu, F.C.; Ho, T.J.; Lai, C.C.; Lin, C.F.; Chen, H.P.
    Cloning, sequencing, expression, and single-step purification of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) from Salmonella typhimurium ATCC 19585 (2005), Protein Expr. Purif., 42, 178-181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.156 gene cobU, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.156 adenosylcobinamide phosphate + GTP Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide-phosphate guanylyltransferase activity adenosylcobinamide-GDP + diphosphate
-
 ir
2.7.1.156 ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide kinase activity adenosylcobinamide phosphate + ADP
-
 ir
2.7.1.156 additional information Salmonella enterica subsp. enterica serovar Typhimurium the enzyme is involved in adenosylcobalamin de novo biosynthesis, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium
-
 ATCC 19585, gene cobU, bifunctional enzyme
-
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium
-
 ATCC 19585
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.156 recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, hydrophobic interaction chromatography on a phenyl resin, and ultrafiltration Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 single-step method based on hydrophobic interaction chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.156 RTP + adenosylcobinamide = adenosylcobinamide phosphate + RDP bifunctional enzyme showing adenosylcobinamide kinase and adenosylcobinamide-phosphate guanylyltransferase activities Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.156 adenosylcobinamide phosphate + GTP adenosylcobinamide-phosphate guanylyltransferase activity Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide-GDP + diphosphate
-
 ir
2.7.1.156 ATP + adenosylcobinamide adenosylcobinamide kinase activity Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
 ir
2.7.1.156 additional information the enzyme is involved in adenosylcobalamin de novo biosynthesis, overview Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate the bifunctional enzyme adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase is involved in the biosynthesis of cobalamin Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.156 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase bifunctional enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 CobU bifunctional enzyme adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase Salmonella enterica subsp. enterica serovar Typhimurium