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Literature summary extracted from

  • Gopalaswamy, R.; Narayanan, P.R.; Narayanan, S.
    Cloning, overexpression, and characterization of a serine/threonine protein kinase pknI from Mycobacterium tuberculosis H37Rv (2004), Protein Expr. Purif., 36, 82-89.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 gene pknI, DNA and amino acid sequence determination and analysis, NaCl-inducible expression of N-terminally His-tagged full-length and cytoplasmic domain of PknI in Escherichia coli strain BL21 in inclusion bodies Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 cytoplasm enzyme contains a cytoplasmic domain Mycobacterium tuberculosis 5737
-
2.7.11.1 membrane
-
 Mycobacterium tuberculosis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Ca2+ can partly substitute for Mn2+, activates Mycobacterium tuberculosis
2.7.11.1 Mg2+ activates Mycobacterium tuberculosis
2.7.11.1 Mn2+ strictly required, activates Mycobacterium tuberculosis
2.7.11.1 additional information no activity with Mg2+ alone, no activity with Cu2+ or Zn2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 55000
-
 x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE Mycobacterium tuberculosis
2.7.11.1 61805
-
 x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Mycobacterium tuberculosis
-
-
-
2.7.11.1 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 recombinant His-tagged full-length and cytoplasmic domain of PknI from Escherichia coli strain BL21 by nickel affinity chromatography Mycobacterium tuberculosis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.11.1 refolding of recombinant enzyme Mycobacterium tuberculosis

Subunits

EC Number Subunits Comment Organism
2.7.11.1 ? x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PknI
-
 Mycobacterium tuberculosis
2.7.11.1 serine/threonine protein kinase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7.4
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.1 Mycobacterium tuberculosis amino acid sequence calculation
-
 5.5