EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.13 | expression in Escherichia coli | Tequintavirus T5 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.13 | 28670 | - |
calculated from nucleotid sequence | Tequintavirus T5 |
2.7.4.13 | 29140 | - |
SDS-PAGE | Tequintavirus T5 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.13 | ATP + deoxynucleoside monophosphate | Tequintavirus T5 | key step in de novo DNA precursor biosynthesis | ADP + deoxynucleoside diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.13 | Tequintavirus T5 | Q6QGP4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.13 | two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases | Tequintavirus T5 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.4.13 | 29 | - |
recombinant protein in sonicated cell exctracts, with dGMP as substrate | Tequintavirus T5 |
2.7.4.13 | 36 | - |
recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate | Tequintavirus T5 |
2.7.4.13 | 40 | - |
recombinant protein after ammonium sulphate precipitation, with dGMP as substrate | Tequintavirus T5 |
2.7.4.13 | 84.2 | - |
homogeneous recombinant enzyme with dCMP as substrate | Tequintavirus T5 |
2.7.4.13 | 153.6 | - |
homogeneous recombinant enzyme with dGMP as substrate | Tequintavirus T5 |
2.7.4.13 | 155 | - |
affinity chromatography eluant of the recombinant protein, with dGMP as substrate | Tequintavirus T5 |
2.7.4.13 | 155 | - |
ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate | Tequintavirus T5 |
2.7.4.13 | 166.8 | - |
homogeneous recombinant enzyme with dTMP as substrate | Tequintavirus T5 |
2.7.4.13 | 232 | - |
homogeneous recombinant enzyme with dAMP as substrate | Tequintavirus T5 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.13 | ATP + dAMP | - |
Tequintavirus T5 | ADP + dADP | - |
? | |
2.7.4.13 | ATP + dCMP | - |
Tequintavirus T5 | ADP + dCDP | - |
? | |
2.7.4.13 | ATP + deoxynucleoside monophosphate | key step in de novo DNA precursor biosynthesis | Tequintavirus T5 | ADP + deoxynucleoside diphosphate | - |
? | |
2.7.4.13 | ATP + dGMP | - |
Tequintavirus T5 | ADP + dGDP | - |
? | |
2.7.4.13 | ATP + dTMP | - |
Tequintavirus T5 | ADP + dTDP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.13 | deoxyribonucleoside monophosphate kinase | - |
Tequintavirus T5 |
2.7.4.13 | dNMP kinase | - |
Tequintavirus T5 |