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Literature summary extracted from

  • Henderson, G.B.; Murgolo, N.J.; Kuriyan, J.; Osapay, K.; Kominos, D.; Berry, A.; Scrutton, N.S.; Hinchliffe, N.W.; Perham, R.N.; Cerami, A.
    Engineering the substrate specificity of glutathione reductase toward that of trypanothione reduction (1991), Proc. Natl. Acad. Sci. USA, 88, 8769-8773.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.7 A18E//N21W/R22N/N101D/K105D/R319A mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold decrease in turnover-number for trypanothione, 9.5fold decrease in KM-value for trypanothione Escherichia coli
1.8.1.7 A18E/N21W/R22N mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.6fold increase in turnover-number for trypanothione, 3fold decrease in KM-value for trypanothione Escherichia coli
1.8.1.7 A18E/N21W/R22N/N101D/K105D mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold increase in turnover-number for trypanothione, 6.7fold decrease in KM-value for trypanothione Escherichia coli
1.8.1.7 N101D/K105D about 2fold increase in KM-value for GSSG, 1.2fold decrease in turnover-number. 1.6fold decrease in turnover-number for trypanothione, 1.15fold increase in KM-value for trypanothione Escherichia coli
1.8.1.7 N21R 1.6fold increase in KM-value for GSSG, 1.4fold decrease in turnover-number for GSSG, 1.2fold decrease in turnover-number for trypanothione, 2.3fold increase in turnover number for trypanothione Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.061
-
GSSG pH 7.5, wild-type enzyme Escherichia coli
1.8.1.7 0.065
-
GSSG pH 7.5 Homo sapiens
1.8.1.7 0.097
-
GSSG pH 7.5, mutant enzyme N21R Escherichia coli
1.8.1.7 0.12
-
GSSG pH 7.5, mutant enzyme N101D/K105D Escherichia coli
1.8.1.7 0.21
-
trypanothione pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D/R319A Escherichia coli
1.8.1.7 0.33
-
trypanothione pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D Escherichia coli
1.8.1.7 0.66
-
trypanothione pH 7.5, mutant enzyme A18E/N21W/R22N Escherichia coli
1.8.1.7 2
-
trypanothione pH 7.5, wild-type enzyme Escherichia coli
1.8.1.7 2.3
-
trypanothione pH 7.5, mutant enzyme N101D/K105D Escherichia coli
1.8.1.7 4.58
-
trypanothione pH 7.5, mutant enzyme N21R Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Escherichia coli
-
-
-
1.8.1.7 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH
-
Escherichia coli glutathione + NADP+
-
?
1.8.1.7 GSSG + NADPH
-
Homo sapiens glutathione + NADP+
-
?
1.8.1.7 trypanothione + NADPH
-
Escherichia coli reduced trypanothione + NADP+
-
?
1.8.1.7 trypanothione + NADPH
-
Homo sapiens reduced trypanothione + NADP+
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.7 0.16
-
trypanothione pH 7.5 Homo sapiens
1.8.1.7 85
-
trypanothione pH 7.5, mutant enzyme N21R Escherichia coli
1.8.1.7 101.7
-
trypanothione pH 7.5, wild-type enzyme Escherichia coli
1.8.1.7 210
-
GSSG pH 7.5 Homo sapiens
1.8.1.7 538.3
-
GSSG pH 7.5, mutant enzyme N21R Escherichia coli
1.8.1.7 733.3
-
GSSG pH 7.5, wild-type enzyme Escherichia coli