EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.7 | A18E//N21W/R22N/N101D/K105D/R319A | mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold decrease in turnover-number for trypanothione, 9.5fold decrease in KM-value for trypanothione | Escherichia coli |
1.8.1.7 | A18E/N21W/R22N | mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.6fold increase in turnover-number for trypanothione, 3fold decrease in KM-value for trypanothione | Escherichia coli |
1.8.1.7 | A18E/N21W/R22N/N101D/K105D | mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold increase in turnover-number for trypanothione, 6.7fold decrease in KM-value for trypanothione | Escherichia coli |
1.8.1.7 | N101D/K105D | about 2fold increase in KM-value for GSSG, 1.2fold decrease in turnover-number. 1.6fold decrease in turnover-number for trypanothione, 1.15fold increase in KM-value for trypanothione | Escherichia coli |
1.8.1.7 | N21R | 1.6fold increase in KM-value for GSSG, 1.4fold decrease in turnover-number for GSSG, 1.2fold decrease in turnover-number for trypanothione, 2.3fold increase in turnover number for trypanothione | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.7 | 0.061 | - |
GSSG | pH 7.5, wild-type enzyme | Escherichia coli | |
1.8.1.7 | 0.065 | - |
GSSG | pH 7.5 | Homo sapiens | |
1.8.1.7 | 0.097 | - |
GSSG | pH 7.5, mutant enzyme N21R | Escherichia coli | |
1.8.1.7 | 0.12 | - |
GSSG | pH 7.5, mutant enzyme N101D/K105D | Escherichia coli | |
1.8.1.7 | 0.21 | - |
trypanothione | pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D/R319A | Escherichia coli | |
1.8.1.7 | 0.33 | - |
trypanothione | pH 7.5, mutant enzyme A18E/N21W/R22N/N101D/K105D | Escherichia coli | |
1.8.1.7 | 0.66 | - |
trypanothione | pH 7.5, mutant enzyme A18E/N21W/R22N | Escherichia coli | |
1.8.1.7 | 2 | - |
trypanothione | pH 7.5, wild-type enzyme | Escherichia coli | |
1.8.1.7 | 2.3 | - |
trypanothione | pH 7.5, mutant enzyme N101D/K105D | Escherichia coli | |
1.8.1.7 | 4.58 | - |
trypanothione | pH 7.5, mutant enzyme N21R | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.7 | Escherichia coli | - |
- |
- |
1.8.1.7 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.7 | GSSG + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
? | |
1.8.1.7 | GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
? | |
1.8.1.7 | trypanothione + NADPH | - |
Escherichia coli | reduced trypanothione + NADP+ | - |
? | |
1.8.1.7 | trypanothione + NADPH | - |
Homo sapiens | reduced trypanothione + NADP+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.7 | 0.16 | - |
trypanothione | pH 7.5 | Homo sapiens | |
1.8.1.7 | 85 | - |
trypanothione | pH 7.5, mutant enzyme N21R | Escherichia coli | |
1.8.1.7 | 101.7 | - |
trypanothione | pH 7.5, wild-type enzyme | Escherichia coli | |
1.8.1.7 | 210 | - |
GSSG | pH 7.5 | Homo sapiens | |
1.8.1.7 | 538.3 | - |
GSSG | pH 7.5, mutant enzyme N21R | Escherichia coli | |
1.8.1.7 | 733.3 | - |
GSSG | pH 7.5, wild-type enzyme | Escherichia coli |