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Literature summary extracted from

  • Zhang, S.; Nichols, S.E.; Dong, J.G.
    Cloning and characterization of two fructokinases from maize (2003), Plant Sci., 165, 1051-1058.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 D-fructose above 0.5 mM; above 1 mM Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.024
-
 ATP pH 8.5, 30°C Zea mays
2.7.1.4 0.109
-
 D-fructose pH 8.5, 30°C Zea mays
2.7.1.4 0.159
-
 D-fructose pH 8.5, 30°C Zea mays
2.7.1.4 0.187
-
 ATP pH 8.5, 30°C Zea mays
2.7.1.4 0.222
-
 UTP pH 8.5, 30°C Zea mays
2.7.1.4 0.491
-
 UTP pH 8.5, 30°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.4 soluble
-
 Zea mays
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 34700
-
 x * 34700, SDS-PAGE Zea mays
2.7.1.4 35500
-
 x * 35500, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Zea mays Q6XZ78 isoform FRK2, recombinant protein with His6-tag
-
2.7.1.4 Zea mays Q6XZ79 isoform FRK1, recombinant protein with His6-tag
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 835
-
 pH 8.5, 30°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Zea mays ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 additional information no substrate: glucose Zea mays ?
-
 ?
2.7.1.4 UTP + D-fructose
-
 Zea mays UDP + D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 ? x * 35500, SDS-PAGE Zea mays
2.7.1.4 ? x * 34700, SDS-PAGE Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.4 494
-
 D-fructose pH 8.5, 30°C Zea mays
2.7.1.4 8897
-
 D-fructose pH 8.5, 30°C Zea mays

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.4 Zea mays calculated
-
 4.87
2.7.1.4 Zea mays calculated
-
 5.34