EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.76 | p-chloromercuribenzoate | 1 mM, complete inhibition | Serratula tinctoria |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.76 | 0.007 | - |
myricetin | 30°C | Serratula tinctoria | |
2.1.1.76 | 0.012 | - |
quercetin | 30°C | Serratula tinctoria | |
2.1.1.76 | 0.013 | - |
kaempferol | 30°C | Serratula tinctoria | |
2.1.1.76 | 0.045 | - |
S-adenosyl-L-methionine | 30°C | Serratula tinctoria |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 31000 | - |
1 * 31000, SDS-PAGE | Serratula tinctoria |
2.1.1.76 | 35000 | - |
gel filtration | Serratula tinctoria |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.76 | Serratula tinctoria | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.76 | partial | Serratula tinctoria |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.1.76 | S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | ordered sequential bi-bi mechanism | Serratula tinctoria |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.76 | leaf | - |
Serratula tinctoria | - |
2.1.1.76 | root | - |
Serratula tinctoria | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 13.8 | - |
- |
Serratula tinctoria |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.76 | S-adenosyl-L-methionine + galangin | 60% of the activity with quercetin | Serratula tinctoria | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + isorhamnetin | 88% of the activity with quercetin | Serratula tinctoria | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + kaempferol | 93% of the activity with quercetin | Serratula tinctoria | S-adenosyl-L-homocysteine + 3-O-methyl-kaempferol | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + myricetin | 46% of the activity with quercetin | Serratula tinctoria | S-adenosyl-L-homocysteine + 3-O-methyl-myricetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + quercetin | - |
Serratula tinctoria | S-adenosyl-L-homocysteine + 3-O-methyl-quercetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + tamarixetin | 85% of the activity with quercetin | Serratula tinctoria | S-adenosyl-L-homocysteine + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.76 | monomer | 1 * 31000, SDS-PAGE | Serratula tinctoria |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 7.6 | - |
- |
Serratula tinctoria |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.1.1.76 | Serratula tinctoria | chromatofocusing | - |
6 |