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Literature summary extracted from

  • Ishiyama, K.; Inoue, E.; Tabuchi, M.; Yamaya, T.; Takahashi, H.
    Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots (2004), Plant Cell Physiol., 45, 1640-1647.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 expression in Escherichia coli, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
6.3.1.2 expression in Escherichia coli, isoenzyme OsGLN1,2 Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.027
-
 NH4+ 30°C, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
6.3.1.2 0.073
-
 NH4+ 30°C, isoenzyme OsGLN1,2 Oryza sativa Japonica Group
6.3.1.2 0.45
-
 ATP 30°C, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
6.3.1.2 0.53
-
 ATP 30°C, isoenzyme OsGLN1,2 Oryza sativa Japonica Group
6.3.1.2 1.9
-
 L-glutamate 30°C, isoenzyme OsGLN1,1 Oryza sativa Japonica Group
6.3.1.2 2.1
-
 L-Glu 30°C, isoenzyme OsGLN1,2 Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+ Oryza sativa Japonica Group serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-glutamate + NH4+ Oryza sativa Japonica Group serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess ADP + phosphate + L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Oryza sativa Japonica Group P14654 isoenzyme OsGLN1,2
-
6.3.1.2 Oryza sativa Japonica Group P14656 isoenzyme OsGLN1,1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 isoenzyme OsGLN1,1 Oryza sativa Japonica Group
6.3.1.2 isoenzyme OsGLN1,2 Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 epidermis OsGLN1,1, under nitrogen-limited condition Oryza sativa Japonica Group
-
6.3.1.2 epidermis OsGLN1,2, under nitrogen-sufficient condition Oryza sativa Japonica Group
-
6.3.1.2 exodermis OsGLN1,1, under nitrogen-limited condition Oryza sativa Japonica Group
-
6.3.1.2 exodermis OsGLN1,2, under nitrogen-sufficient condition Oryza sativa Japonica Group
-
6.3.1.2 root OsGLN1,1 is expressed in epidermis and exodermis under nitrogen-limited condition. Within the central cylinder of the elongating zone the enzyme is induced by ammonium Oryza sativa Japonica Group
-
6.3.1.2 root OsGLN1,2 is expressed in epidermis and exodermis under nitrogen-sufficient condition. Within the central cylinder of the elongating zone the enzyme is induced by ammonium Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Oryza sativa Japonica Group ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+ serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess Oryza sativa Japonica Group ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-glutamate + NH4+
-
 Oryza sativa Japonica Group ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH4+ serves for assimilation of ammonium in rice root, and ameliorates the toxic effect of ammonium excess Oryza sativa Japonica Group ADP + phosphate + L-glutamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 OsGLN1,1
-
 Oryza sativa Japonica Group
6.3.1.2 OsGLN1,2
-
 Oryza sativa Japonica Group