Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fukao, T.; Shintaku, H.; Kusubae, R.; Zhang, G.X.; Nakamura, K.; Kondo, M.; Kondo, N.
    Patients homozygous for the T435N mutation of succinyl-CoA:3-ketoacid CoA Transferase (SCOT) do not show permanent ketosis (2004), Pediatr. Res., 56, 858-863.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.8.3.5 medicine patients homozygous for T435N mutation of SCOT do not show permanent ketosis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.5 expression in SCOT-deficient SV 40-transformed fibroblasts Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.3.5 C456F no activity Homo sapiens
2.8.3.5 T435N retains significant residual SCOT activities Homo sapiens
2.8.3.5 V133E no activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate Homo sapiens
-
 succinate + acetoacetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.5 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.5 additional information
-
 mutant T435N shows 20% of wild type activity at 39.5°C, 25% at 37°C, and 50% at 30°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate
-
 Homo sapiens succinate + acetoacetyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.3.5 SCOT
-
 Homo sapiens
2.8.3.5 succinyl-CoA:3-ketoacid CoA transferase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.3.5 42
-
 incubation of wild type at 42°C for 10 min does not affect enzyme activity, incubation of mutant T435N at 42°C for 10 min reduces enzyme activity to 50% Homo sapiens
2.8.3.5 55
-
 incubation of wild type at 55°C for 10 min reduces enzyme activity to 20%, incubation of T435N at 55°C results in more rapid inactivation of enzyme activity (below 10% in a 4-min incubation) Homo sapiens