EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.6 | expressed as GST-fusion protein in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.6 | H117A | no effect on nuclease activity | Mycobacterium tuberculosis |
2.7.4.6 | K10A | nuclease activity completely abolished | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.6 | ATP | inhibition of nuclease activity | Mycobacterium tuberculosis | |
2.7.4.6 | GDP | competitive inhibition of nuclease activity | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.4.6 | extracellular | enzyme is secreted into the culture medium | Mycobacterium tuberculosis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.6 | Mg2+ | required for DNA cleavage reaction | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.6 | ATP + dNDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
2.7.4.6 | ATP + dNDP | Mycobacterium tuberculosis H37Rv | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | ADP + dNTP | - |
? | |
2.7.4.6 | ATP + NDP | Mycobacterium tuberculosis | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | ADP + NTP | - |
? | |
2.7.4.6 | ATP + NDP | Mycobacterium tuberculosis H37Rv | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | ADP + NTP | - |
? | |
2.7.4.6 | additional information | Mycobacterium tuberculosis | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | ? | - |
? | |
2.7.4.6 | additional information | Mycobacterium tuberculosis H37Rv | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.6 | Mycobacterium tuberculosis | P9WJH7 | - |
- |
2.7.4.6 | Mycobacterium tuberculosis H37Rv | P9WJH7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.6 | recombinant protein using GST-tag | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.6 | ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis | ADP + dNTP | - |
? | |
2.7.4.6 | ATP + dNDP | contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates | Mycobacterium tuberculosis H37Rv | ADP + dNTP | - |
? | |
2.7.4.6 | ATP + GDP | - |
Mycobacterium tuberculosis | ADP + GTP | - |
? | |
2.7.4.6 | ATP + GDP | - |
Mycobacterium tuberculosis H37Rv | ADP + GTP | - |
? | |
2.7.4.6 | ATP + NDP | - |
Mycobacterium tuberculosis | ADP + NTP | - |
? | |
2.7.4.6 | ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | Mycobacterium tuberculosis | ADP + NTP | - |
? | |
2.7.4.6 | ATP + NDP | - |
Mycobacterium tuberculosis H37Rv | ADP + NTP | - |
? | |
2.7.4.6 | ATP + NDP | contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ | Mycobacterium tuberculosis H37Rv | ADP + NTP | - |
? | |
2.7.4.6 | additional information | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | Mycobacterium tuberculosis | ? | - |
? | |
2.7.4.6 | additional information | catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.6 | hexamer | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.6 | 1.9 | - |
GDP | competitive inhibition of nuclease activity | Mycobacterium tuberculosis |