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Literature summary extracted from

  • Kumar, P.; Verma, A.; Saini, A.K.; Chopra, P.; Chakraborti, P.K.; Singh, Y.; Chowdhury, S.
    Nucleoside diphosphate kinase from Mycobacterium tuberculosis cleaves single strand DNA within the human c-myc promoter in an enzyme-catalyzed reaction (2005), Nucleic Acids Res., 33, 2707-2714.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expressed as GST-fusion protein in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 H117A no effect on nuclease activity Mycobacterium tuberculosis
2.7.4.6 K10A nuclease activity completely abolished Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 ATP inhibition of nuclease activity Mycobacterium tuberculosis
2.7.4.6 GDP competitive inhibition of nuclease activity Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 extracellular enzyme is secreted into the culture medium Mycobacterium tuberculosis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+ required for DNA cleavage reaction Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + dNDP Mycobacterium tuberculosis contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates ADP + dNTP
-
 ?
2.7.4.6 ATP + dNDP Mycobacterium tuberculosis H37Rv contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates ADP + dNTP
-
 ?
2.7.4.6 ATP + NDP Mycobacterium tuberculosis contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ ADP + NTP
-
 ?
2.7.4.6 ATP + NDP Mycobacterium tuberculosis H37Rv contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ ADP + NTP
-
 ?
2.7.4.6 additional information Mycobacterium tuberculosis catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments ?
-
 ?
2.7.4.6 additional information Mycobacterium tuberculosis H37Rv catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Mycobacterium tuberculosis P9WJH7
-
-
2.7.4.6 Mycobacterium tuberculosis H37Rv P9WJH7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant protein using GST-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + dNDP contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates Mycobacterium tuberculosis ADP + dNTP
-
 ?
2.7.4.6 ATP + dNDP contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates Mycobacterium tuberculosis H37Rv ADP + dNTP
-
 ?
2.7.4.6 ATP + GDP
-
 Mycobacterium tuberculosis ADP + GTP
-
 ?
2.7.4.6 ATP + GDP
-
 Mycobacterium tuberculosis H37Rv ADP + GTP
-
 ?
2.7.4.6 ATP + NDP
-
 Mycobacterium tuberculosis ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ Mycobacterium tuberculosis ADP + NTP
-
 ?
2.7.4.6 ATP + NDP
-
 Mycobacterium tuberculosis H37Rv ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ Mycobacterium tuberculosis H37Rv ADP + NTP
-
 ?
2.7.4.6 additional information catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments Mycobacterium tuberculosis ?
-
 ?
2.7.4.6 additional information catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.6 1.9
-
 GDP competitive inhibition of nuclease activity Mycobacterium tuberculosis