EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.113 | medicine | the enzyme activates a number of medically important nucleoside analogues | Homo sapiens |
2.7.1.145 | medicine | the enzyme activates a number of medically important nucleoside analogues | Drosophila melanogaster |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.113 | purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 7 mg/ml protein, 5 mM MgCl2, and 5 mM ATP, mixed with an equal volume of cyrstallization solution containing 0.1 M Na-citrate, pH 6.5, 0.2 M ammonium acetate, and 30% w/v PEG 4000, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement method | Homo sapiens |
2.7.1.145 | purified recombinant enzyme, hanging drop vapour diffusion method, enzyme solution containing 10 mg/ml protein and 10 mM deoxycytidine, mixed with an equal volume of cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 20% w/v PEG 5000, and 8-10% v/v PEG 400, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement method | Drosophila melanogaster |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.145 | ATP stabilizes the enzyme structure | Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.113 | Mg2+ | - |
Homo sapiens | |
2.7.1.145 | Mg2+ | - |
Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.113 | Homo sapiens | Q16854 | - |
- |
2.7.1.145 | Drosophila melanogaster | Q9XZT6 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.113 | ATP + deoxyguanosine = ADP + dGMP | substrate interactions and binding mechanism, and active site structure | Homo sapiens | |
2.7.1.145 | ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate | substrate binding and interactions | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.113 | ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.113 | additional information | the enzyme is highly specific for purine substrates, Arg118 is responsible for the substrate specificity | Homo sapiens | ? | - |
? | |
2.7.1.145 | ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | additional information | the enzyme shows broad substrate specificity probably due to a wide substrate binding cleft | Drosophila melanogaster | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.113 | DGK | - |
Homo sapiens |
2.7.1.145 | dNK | - |
Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.113 | ATP | - |
Homo sapiens | |
2.7.1.145 | ATP | stabilizes the enzyme structure, binding structure, Q111 and Arg118 are involved | Drosophila melanogaster |