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Literature summary extracted from

  • Vedavathi, M.; Girish, K.S.; Kumar, M.K.
    Isolation and characterization of cytosolic alanine aminotransferase isoforms from starved rat liver (2004), Mol. Cell. Biochem., 267, 13-23.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 Aminooxyacetate complete inhibition, both isoforms Rattus norvegicus
2.6.1.2 Glutarate about 70% inhibition, both isoforms Rattus norvegicus
2.6.1.2 HgCl2 almost complete inhibition, both isoforms Rattus norvegicus
2.6.1.2 hydroxylamine complete inhibition, both isoforms Rattus norvegicus
2.6.1.2 Maleate about 70% inhibition, both isoforms Rattus norvegicus
2.6.1.2 o-phenanthroline weak Rattus norvegicus
2.6.1.2 p-hydroxymercuribenzoate complete inhibition, both isoforms Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.2 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 17700
-
 x * 17700, isoform AlaAT I, x * 112200, isoform AlaAT II, SDS-PAGE Rattus norvegicus
2.6.1.2 18370
-
 isoform AlaAT I, gel filtration Rattus norvegicus
2.6.1.2 112200
-
 x * 17700, isoform AlaAT I, x * 112200, isoform AlaAT II, SDS-PAGE Rattus norvegicus
2.6.1.2 114800
-
 isoform AlaAT II, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Rattus norvegicus
-
 male animals, under starvation, isoforms AlaAT I and AlaAT II
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 30.58
-
 isoform AlaAT I Rattus norvegicus
2.6.1.2 43.3
-
 isoform AlaAT II Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 DL-alanine + 2-oxoglutarate 63-70% of the activity with L-alanine Rattus norvegicus pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Rattus norvegicus pyruvate + L-glutamate
-
 ?
2.6.1.2 L-aspartate + 2-oxoglutarate 6-8% of the activity with L-alanine Rattus norvegicus 2-oxobutan-1,4-dioate + L-glutamate
-
 ?
2.6.1.2 L-leucine + 2-oxoglutarate 8% of the activity with L-alanine Rattus norvegicus 2-oxo-4-methylpentanoate + L-glutamate
-
 ?
2.6.1.2 L-tyrosine + 2-oxoglutarate 12-14% of the activity with L-alanine Rattus norvegicus 2-deamino-2-oxotyrosine + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.2 monomer x * 17700, isoform AlaAT I, x * 112200, isoform AlaAT II, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.2 AlaAT
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 55
-
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 8.5
-
-
 Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.2 Rattus norvegicus isoform AlaAT I, isoelectric focusing
-
 4.2
2.6.1.2 Rattus norvegicus isoform AlaAT II, isoelectric focusing
-
 5.3