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Literature summary extracted from

  • Oza, S.L.; Shaw, M.P.; Wyllie, S.; Fairlamb, A.H.
    Trypanothione biosynthesis in Leishmania major (2005), Mol. Biochem. Parasitol., 139, 107-116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.8 gene GSPS, a pseudogene with 2 frame shift mutations and 2 stop codons, inactive enzyme, phylogenetic analysis Leishmania major
6.3.1.9 gene TRYS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.9 glutathione substrate inhibition Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.9 additional information
-
 additional information hyperbolic kinetics, steady-state kinetics Leishmania major
6.3.1.9 0.04
-
 glutathionylspermidine pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major
6.3.1.9 0.063
-
 MgATP2- pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major
6.3.1.9 0.089
-
 glutathione pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major
6.3.1.9 0.94
-
 spermidine pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.9 cytosol
-
 Leishmania major 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.9 (NH4)2SO4 optimal up to 50 mM Leishmania major
6.3.1.9 KCl optimal up to 50 mM Leishmania major
6.3.1.9 Mg2+ as MgATP2- Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.9 2 glutathione + spermidine + 2 ATP Leishmania major trypanothione synthase overall reaction N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Leishmania major trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Leishmania major trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 additional information Leishmania major phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Leishmania major AJ748279 inactive gene GSPS; inactive gene GSPS
-
6.3.1.9 Leishmania major Q711P7 gene TRYS; gene TRYS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.9 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, and ion exchange chromatography Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.9 2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction Leishmania major N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP ATP in form of MgATP2- Leishmania major glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 additional information phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome Leishmania major ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.8 GspS
-
 Leishmania major
6.3.1.9 TryS
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.9 25
-
 assay at Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.9 2
-
 glutathione pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.9 8.5
-
 with substrates glutathionylspermidine and spermidine Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.9 ATP as MgATP2-, the enzyme is specific for ATP and shows only negligible activity with other NTPs Leishmania major

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.1.9 1
-
 glutathione pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major