Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kasekarn, W.; Sirawaraporn, R.; Chahomchuen, T.; Cowman, A.F.; Sirawaraporn, W.
    Molecular characterization of bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium falciparum (2004), Mol. Biochem. Parasitol., 137, 43-53.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 expressed under the control of the T5 promoter in a DHPS-deficient Escherichia coli strain Plasmodium falciparum
2.7.6.3 expression in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 dapsone
-
 Plasmodium falciparum
2.5.1.15 Guanidine HCl 0.25 M, 50% inhibition Plasmodium falciparum
2.5.1.15 KCl above 0.4 M Plasmodium falciparum
2.5.1.15 NaCl above 0.2 M Plasmodium falciparum
2.5.1.15 p-Aminosalicylic acid
-
 Plasmodium falciparum
2.5.1.15 sulfacetamide
-
 Plasmodium falciparum
2.5.1.15 sulfachloropyridazine
-
 Plasmodium falciparum
2.5.1.15 Sulfadiazine
-
 Plasmodium falciparum
2.5.1.15 sulfadimethoxine
-
 Plasmodium falciparum
2.5.1.15 sulfadoxine
-
 Plasmodium falciparum
2.5.1.15 sulfamerazine
-
 Plasmodium falciparum
2.5.1.15 sulfamethoxazole
-
 Plasmodium falciparum
2.5.1.15 sulfamethoxypyridazine
-
 Plasmodium falciparum
2.5.1.15 sulfamoxole
-
 Plasmodium falciparum
2.5.1.15 sulfanilamide
-
 Plasmodium falciparum
2.5.1.15 sulfapyridine
-
 Plasmodium falciparum
2.5.1.15 sulfaquinoxaline
-
 Plasmodium falciparum
2.5.1.15 sulfathiazole
-
 Plasmodium falciparum
2.5.1.15 sulfisoxazole
-
 Plasmodium falciparum
2.5.1.15 Urea 0.9 M, 50% inhibition Plasmodium falciparum
2.7.6.3 Guanidine-HCl 0.25 M, 50% inhibitioin Plasmodium falciparum
2.7.6.3 KCl activation at 0.4 M, inhibition at higher concentration Plasmodium falciparum
2.7.6.3 NaCl activation at 0.2 M, inhibition at higher concentration Plasmodium falciparum
2.7.6.3 sulfadoxine competitive, moderately potent Plasmodium falciparum
2.7.6.3 sulfathiazole competitive Plasmodium falciparum
2.7.6.3 Urea 0.9 M, 50% inhibition Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.00122
-
 2-Amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
-
 Plasmodium falciparum
2.5.1.15 0.00125
-
 4-Aminobenzoate
-
 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.15 KCl 0.4 M activates Plasmodium falciparum
2.5.1.15 Mg2+ dependent on Plasmodium falciparum
2.5.1.15 NaCl 0.2 M activates Plasmodium falciparum
2.7.6.3 KCl activation at 0.4 M, inhibition at higher concentration Plasmodium falciparum
2.7.6.3 Mg2+ required Plasmodium falciparum
2.7.6.3 NaCl activation at 0.2 M, inhibition at higher concentration Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 83000
-
 bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, gel filtration Plasmodium falciparum
2.7.6.3 83000
-
 4 * 83000, SDS-PAGE Plasmodium falciparum
2.7.6.3 330000
-
 gel filtration Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Plasmodium falciparum
-
-
-
2.7.6.3 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase Plasmodium falciparum
2.7.6.3
-
 Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.15 0.0106
-
-
 Plasmodium falciparum

Storage Stability

EC Number Storage Stability Organism
2.5.1.15 -20°C and -80°C stable for 3 months in 20 mM Tris–HCl, pH 8.0 buffer containing 10% or 20% glycerol Plasmodium falciparum
2.5.1.15 -20°C or -80°C, in buffer without glycerol, 30% and 50% of the enzyme activity is lost when stored for 6 months Plasmodium falciparum
2.7.6.3 -20°C or -80°C for 3 months in 20 mM Tris-HCl, pH 8.0 buffer containing 10 or 20% glycerol, no loss of activity. In buffer without glycerol, 30 and 50% of the enzyme activity are lost when stored for 6 months at -80°C and -20°C, respectively Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + 4-aminobenzoate
-
 Plasmodium falciparum 7,8-dihydropteroate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.15 tetramer
-
 Plasmodium falciparum
2.7.6.3 tetramer 4 * 83000, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.5.1.15 hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme Plasmodium falciparum
2.5.1.15 pfPPK-DHPS recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase Plasmodium falciparum
2.7.6.3 hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.15 37 45
-
 Plasmodium falciparum
2.7.6.3 37 45
-
 Plasmodium falciparum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.15 30 55 30°C: about 40% of maximal activity, 55°C: about 10% of maximal activity Plasmodium falciparum
2.7.6.3 30 45 30°C: about 40% of maximal activity, 37-45°C: optimum Plasmodium falciparum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.15 55
-
 inactivation above Plasmodium falciparum
2.7.6.3 55
-
 complete inactivation Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.15 10
-
-
 Plasmodium falciparum
2.7.6.3 10
-
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.15 8.5 10.5 pH 8.5: about 40% of maximal activity, pH 10.5: about 85% of maximal activity Plasmodium falciparum
2.7.6.3 8.5 10.5 pH 8.5: about 40% of maximal activity, pH 10.5: about 90% of maximal activity Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 0.0006
-
 sulfathiazole
-
 Plasmodium falciparum
2.5.1.15 0.001
-
 dapsone
-
 Plasmodium falciparum
2.5.1.15 0.0015
-
 sulfachloropyridazine
-
 Plasmodium falciparum
2.5.1.15 0.0016
-
 sulfaquinoxaline
-
 Plasmodium falciparum
2.5.1.15 0.0018
-
 sulfadimethoxine
-
 Plasmodium falciparum
2.5.1.15 0.0019
-
 sulfapyridine
-
 Plasmodium falciparum
2.5.1.15 0.0027
-
 sulfamoxole
-
 Plasmodium falciparum
2.5.1.15 0.0033
-
 sulfamethoxypyridazine
-
 Plasmodium falciparum
2.5.1.15 0.0044
-
 sulfamethoxazole
-
 Plasmodium falciparum
2.5.1.15 0.0048
-
 sulfamerazine
-
 Plasmodium falciparum
2.5.1.15 0.0068
-
 Sulfadiazine
-
 Plasmodium falciparum
2.5.1.15 0.0076
-
 sulfisoxazole
-
 Plasmodium falciparum
2.5.1.15 0.0082
-
 p-Aminosalicylic acid
-
 Plasmodium falciparum
2.5.1.15 0.011
-
 sulfadoxine
-
 Plasmodium falciparum
2.5.1.15 0.0437
-
 sulfanilamide
-
 Plasmodium falciparum
2.5.1.15 0.0941
-
 sulfacetamide
-
 Plasmodium falciparum
2.7.6.3 0.011
-
 sulfadoxine
-
 Plasmodium falciparum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.15 Plasmodium falciparum recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase
-
 7.11
2.7.6.3 Plasmodium falciparum calulation from amino acid sequence
-
 7.1