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Literature summary extracted from

  • Dunn, M.F.; Araiza, G.; Mora, J.
    Biochemical characterization of a Rhizobium etli monovalent cation-stimulated acyl-coenzyme A carboxylase with a high substrate specificity constant for propionyl-coenzyme A (2004), Microbiology, 150, 399-406.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.3 NH4+ 20 mM, activates, monovalent cation required Rhizobium etli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 additional information
-
 additional information kinetics Rhizobium etli
6.4.1.2 0.105
-
 ATP pH 8.0 Rhizobium etli
6.4.1.2 0.144
-
 propionyl-CoA pH 8.0 Rhizobium etli
6.4.1.2 0.392
-
 acetyl-CoA pH 8.0 Rhizobium etli
6.4.1.2 0.645
-
 HCO3- pH 8.0 Rhizobium etli
6.4.1.2 0.83
-
 Mg2+ pH 8.0 Rhizobium etli
6.4.1.3 0.064
-
 propanoyl-CoA
-
 Rhizobium etli
6.4.1.3 0.144
-
 butyryl-CoA
-
 Rhizobium etli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Cs+ dependent on Rhizobium etli
6.4.1.2 K+ dependent on Rhizobium etli
6.4.1.2 Mg2+ absolutely required for activity Rhizobium etli
6.4.1.2 NH4+ dependent on Rhizobium etli
6.4.1.3 Cs+ 20 mM, activates, monovalent cation required Rhizobium etli
6.4.1.3 K+ 20 mM, activates, monovalent cation required Rhizobium etli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 45000
-
 x * 74000, alpha-subunit, + x * 45000, beta-subunit, SDS-PAGE Rhizobium etli
6.4.1.2 74000
-
 x * 74000, alpha-subunit, + x * 45000, beta-subunit, SDS-PAGE Rhizobium etli
6.4.1.2 620000
-
 gel filtration Rhizobium etli
6.4.1.3 45000
-
 alpha6beta6, 6 * 74000 + 6 * 45000, SDS-PAGE Rhizobium etli
6.4.1.3 74000
-
 alpha6beta6, 6 * 74000 + 6 * 45000, SDS-PAGE Rhizobium etli
6.4.1.3 620000
-
 gel filtration Rhizobium etli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Rhizobium etli
-
 ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Rhizobium etli 12-53
-
 ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + butyryl-CoA + HCO3- + H+ Rhizobium etli
-
 ADP + glutaryl-CoA + phosphate
-
 ?
6.4.1.2 ATP + butyryl-CoA + HCO3- + H+ Rhizobium etli 12-53
-
 ADP + glutaryl-CoA + phosphate
-
 ?
6.4.1.2 ATP + propionyl-CoA + HCO3- + H+ Rhizobium etli participation in an anaplerotic pathway causes preference for propionyl-CoA as substrate ADP + succinyl-CoA + phosphate
-
 ?
6.4.1.2 additional information Rhizobium etli the enzyme is bifunctional exhibiting both acetyl-CoA carboxylase and propionyl-CoA carboxylase, EC 6.4.1.3, activities ?
-
 ?
6.4.1.2 additional information Rhizobium etli 12-53 the enzyme is bifunctional exhibiting both acetyl-CoA carboxylase and propionyl-CoA carboxylase, EC 6.4.1.3, activities ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Rhizobium etli
-
 the activities of EC 6.4.1.2 and EC 6.4.1.3 are located on a single protein
-
6.4.1.2 Rhizobium etli 12-53
-
 the activities of EC 6.4.1.2 and EC 6.4.1.3 are located on a single protein
-
6.4.1.3 Agrobacterium tumefaciens
-
 IAM13299
-
6.4.1.3 Agrobacterium tumefaciens IAM13299
-
 IAM13299
-
6.4.1.3 Azorhizobium caulinodans
-
 ORS571
-
6.4.1.3 Azorhizobium caulinodans ORS 571
-
 ORS571
-
6.4.1.3 Bradyrhizobium japonicum
-
 USDA110
-
6.4.1.3 Bradyrhizobium japonicum USDA 110
-
 USDA110
-
6.4.1.3 Mesorhizobium huakuii
-
 CCBAU2609
-
6.4.1.3 Mesorhizobium huakuii CCBAU2609
-
 CCBAU2609
-
6.4.1.3 Mesorhizobium loti
-
 NZP2037
-
6.4.1.3 Mesorhizobium loti NZP2037
-
 NZP2037
-
6.4.1.3 Rhizobium etli
-
-
-
6.4.1.3 Rhizobium leguminosarum bv. viciae
-
 MNF300
-
6.4.1.3 Rhizobium leguminosarum bv. viciae MNF300
-
 MNF300
-
6.4.1.3 Rhizobium tropici
-
-
-
6.4.1.3 Sinorhizobium fredii
-
 USDA205
-
6.4.1.3 Sinorhizobium fredii USDA205
-
 USDA205
-
6.4.1.3 Sinorhizobium meliloti
-
 Rm1021
-
6.4.1.3 Sinorhizobium meliloti Rm1021
-
 Rm1021
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 478fold by ammonium sulfate fractionation, ion exchange chromatography, ultrafiltration, and gel filtration Rhizobium etli
6.4.1.3
-
 Rhizobium etli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.4.1.2 ATP + acetyl-CoA + hydrogencarbonate = ADP + phosphate + malonyl-CoA the enzyme is bifunctional exhibiting both acetyl-CoA carboxylase and propionyl-CoA carboxylase, EC 6.4.1.3, activities Rhizobium etli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 0.0004
-
 acetyl-CoA carboxylase activity, strain CE3 Rhizobium etli
6.4.1.2 0.0005
-
 acetyl-CoA carboxylase activity, strain 12-53 Rhizobium etli
6.4.1.2 0.001
-
 acetyl-CoA carboxylase activity, strain CFNX218 Rhizobium etli
6.4.1.2 0.006
-
 propionyl-CoA carboxylase activity, strain CE3 Rhizobium etli
6.4.1.2 0.0064
-
 propionyl-CoA carboxylase activity, strain 12-53 Rhizobium etli
6.4.1.2 0.0078
-
 propionyl-CoA carboxylase activity, strain CFNX218 Rhizobium etli
6.4.1.2 7.27
-
 purified enzyme, substrate propionyl-CoA Rhizobium etli
6.4.1.3 0.00186
-
 cell culture Agrobacterium tumefaciens
6.4.1.3 0.00199
-
 cell culture Mesorhizobium loti
6.4.1.3 0.00442
-
 cell culture Sinorhizobium fredii
6.4.1.3 0.00459
-
 cell culture Rhizobium leguminosarum bv. viciae
6.4.1.3 0.00483
-
 cell culture Mesorhizobium huakuii
6.4.1.3 0.00535
-
 cell culture Rhizobium tropici
6.4.1.3 0.00673
-
 cell culture Sinorhizobium meliloti
6.4.1.3 0.01177
-
 cell culture Azorhizobium caulinodans
6.4.1.3 0.0248
-
 cell culture Bradyrhizobium japonicum
6.4.1.3 7.27
-
-
 Rhizobium etli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Rhizobium etli ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Rhizobium etli 12-53 ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + butyryl-CoA + HCO3- + H+
-
 Rhizobium etli ADP + glutaryl-CoA + phosphate
-
 ?
6.4.1.2 ATP + butyryl-CoA + HCO3- + H+
-
 Rhizobium etli 12-53 ADP + glutaryl-CoA + phosphate
-
 ?
6.4.1.2 ATP + propionyl-CoA + HCO3- + H+ highly preferred substrate Rhizobium etli ADP + succinyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + propionyl-CoA + HCO3- + H+ participation in an anaplerotic pathway causes preference for propionyl-CoA as substrate Rhizobium etli ADP + succinyl-CoA + phosphate
-
 ?
6.4.1.2 additional information the enzyme is bifunctional exhibiting both acetyl-CoA carboxylase and propionyl-CoA carboxylase, EC 6.4.1.3, activities Rhizobium etli ?
-
 ?
6.4.1.2 additional information the enzyme is bifunctional exhibiting both acetyl-CoA carboxylase and propionyl-CoA carboxylase, EC 6.4.1.3, activities Rhizobium etli 12-53 ?
-
 ?
6.4.1.3 ATP + butyryl-CoA + HCO3-
-
 Rhizobium etli ADP + phosphate + ?
-
 ?
6.4.1.3 ATP + butyryl-CoA + HCO3-
-
 Rhizobium etli 12-53 ADP + phosphate + ?
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Sinorhizobium meliloti ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Bradyrhizobium japonicum ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Rhizobium etli ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Agrobacterium tumefaciens ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Sinorhizobium fredii ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Mesorhizobium loti ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Azorhizobium caulinodans ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Rhizobium tropici ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Rhizobium leguminosarum bv. viciae ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Mesorhizobium huakuii ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Rhizobium leguminosarum bv. viciae MNF300 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Azorhizobium caulinodans ORS 571 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Agrobacterium tumefaciens IAM13299 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Bradyrhizobium japonicum USDA 110 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Sinorhizobium fredii USDA205 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Sinorhizobium meliloti Rm1021 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Rhizobium etli 12-53 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Mesorhizobium loti NZP2037 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
 Mesorhizobium huakuii CCBAU2609 ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 oligomer x * 74000, alpha-subunit, + x * 45000, beta-subunit, SDS-PAGE Rhizobium etli
6.4.1.3 dodecamer alpha6beta6, 6 * 74000 + 6 * 45000, SDS-PAGE Rhizobium etli

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 ACC
-
 Rhizobium etli
6.4.1.2 More c.f. EC 6.4.1.3 Rhizobium etli
6.4.1.3 PCC
-
 Sinorhizobium meliloti
6.4.1.3 PCC
-
 Bradyrhizobium japonicum
6.4.1.3 PCC
-
 Agrobacterium tumefaciens
6.4.1.3 PCC
-
 Sinorhizobium fredii
6.4.1.3 PCC
-
 Mesorhizobium loti
6.4.1.3 PCC
-
 Azorhizobium caulinodans
6.4.1.3 PCC
-
 Rhizobium tropici
6.4.1.3 PCC
-
 Rhizobium leguminosarum bv. viciae
6.4.1.3 PCC
-
 Mesorhizobium huakuii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 8
-
 assay at Rhizobium etli

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 ATP dependent on Rhizobium etli
6.4.1.2 biotin dependent on Rhizobium etli
6.4.1.3 biotin dependent on Rhizobium etli