Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aramori, I.; Fukagawa, M.; Tsumura, M.; Iwami, M.; Ono, H.; Ishitani, Y.; Koji, H.; Kohsaka, M.; Ueda, Y.; Imanaka, H.
    Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases (1992), J. Ferment. Bioeng., 73, 185-192.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.93 7-aminocephalosporanate competitive Brevibacillus laterosporus
3.5.1.93 7-aminocephalosporanate competitive Brevundimonas diminuta
3.5.1.93 7-aminocephalosporanate competitive Pseudomonas sp.
3.5.1.93 EDTA 5 mM, 27% inhibition Brevundimonas diminuta
3.5.1.93 Glutarate competitive Brevibacillus laterosporus
3.5.1.93 Glutarate competitive Brevundimonas diminuta
3.5.1.93 Glutarate competitive Pseudomonas sp.
3.5.1.93 PCMB 92% inhibition at 1 mM, 96% inhibition at 1 mM Brevundimonas diminuta
3.5.1.93 PCMB 5 mM, 10% inhibition Pseudomonas sp.
3.5.1.93 PMSF 10% inhibition at 1 mM, 22% inhibition at 5 mM Brevibacillus laterosporus
3.5.1.93 PMSF 5 mM, 38% inhibition Brevundimonas diminuta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.93 2.1
-
 glutaryl-7-aminocephalosporanic acid
-
 Pseudomonas sp.
3.5.1.93 2.6
-
 glutaryl-7-aminocephalosporanic acid
-
 Brevundimonas diminuta
3.5.1.93 3.2
-
 glutaryl-7-aminocephalosporanic acid
-
 Brevibacillus laterosporus
3.5.1.93 4.8
-
 cephalosporin C
-
 Brevundimonas diminuta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.93 26000
-
 1 * 26000 + 1 * 58000, SDS-PAGE Brevundimonas diminuta
3.5.1.93 28000
-
 1 * 28000 + 1 * 61000, SDS-PAGE Pseudomonas sp.
3.5.1.93 50000
-
 gel filtration Brevibacillus laterosporus
3.5.1.93 54000
-
 gel filtration Pseudomonas sp.
3.5.1.93 54000
-
 gel filtration Brevundimonas diminuta
3.5.1.93 58000
-
 1 * 26000 + 1 * 58000, SDS-PAGE Brevundimonas diminuta
3.5.1.93 61000
-
 1 * 28000 + 1 * 61000, SDS-PAGE Pseudomonas sp.
3.5.1.93 70000
-
 1 * 70000, SDS-PAGE Brevibacillus laterosporus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Brevibacillus laterosporus
-
 J1
-
3.5.1.93 Brevibacillus laterosporus J1
-
 J1
-
3.5.1.93 Brevundimonas diminuta
-
 N176
-
3.5.1.93 Brevundimonas diminuta N176
-
 N176
-
3.5.1.93 Pseudomonas sp.
-
 A14, recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.93 recombinant enzyme Pseudomonas sp.
3.5.1.93 recombinant enzyme Brevibacillus laterosporus
3.5.1.93 recombinant enzyme Brevundimonas diminuta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.93 5.3
-
-
 Brevibacillus laterosporus
3.5.1.93 7.1
-
-
 Pseudomonas sp.
3.5.1.93 100
-
-
 Brevundimonas diminuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 adipyl-7-aminocephalosporanic acid + H2O activity is 19% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta 7-aminocephalosporanate + adipate
-
 ?
3.5.1.93 adipyl-7-aminocephalosporanic acid + H2O activity is 19% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta N176 7-aminocephalosporanate + adipate
-
 ?
3.5.1.93 adipyl-7-aminocephalosporanic acid + H2O activity is 0.56% of the activity with glutaryl-7-aminocephalosporanic acid Pseudomonas sp. 7-aminocephalosporanic acid + adipate
-
 ?
3.5.1.93 adipyl-7-aminocephalosporanic acid + H2O activity is 68% of the activity with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus 7-aminocephalosporanic acid + adipate
-
 ?
3.5.1.93 adipyl-7-aminocephalosporanic acid + H2O activity is 68% of the activity with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus J1 7-aminocephalosporanic acid + adipate
-
 ?
3.5.1.93 cephalosporin C + H2O weak activity Pseudomonas sp. cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O weak activity Brevibacillus laterosporus cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O activity is 4% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O activity is 4% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta N176 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O weak activity Brevibacillus laterosporus J1 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 D-glutamyl-7-aminocephalosporanic acid + H2O activity is 0.85% of the activity with glutaryl-7-aminocephalosporanic acid Pseudomonas sp. 7-aminocephalosporanic acid + D-glutamate
-
 ?
3.5.1.93 D-glutamyl-7-aminocephalosporanic acid + H2O activity is 0.55% of the activity with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus 7-aminocephalosporanic acid + glutamate
-
 ?
3.5.1.93 D-glutamyl-7-aminocephalosporanic acid + H2O activity is 0.55% of the activity with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus J1 7-aminocephalosporanic acid + glutamate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O rate of synthesis is fold slower than rate of synthesis Brevundimonas diminuta 7-aminocephalosporanate + glutarate
-
 r
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O rate of synthesis is slower than rate of hydrolysis Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 r
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O rate of synthesis is slower than rate of hydrolysis Brevibacillus laterosporus 7-aminocephalosporanate + glutarate
-
 r
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O rate of synthesis is fold slower than rate of synthesis Brevundimonas diminuta N176 7-aminocephalosporanate + glutarate
-
 r
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O rate of synthesis is slower than rate of hydrolysis Brevibacillus laterosporus J1 7-aminocephalosporanate + glutarate
-
 r
3.5.1.93 N-acetyl cephalosporin C + H2O activity is 1% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta acetate + cephalosporin C
-
 ?
3.5.1.93 N-acetyl cephalosporin C + H2O activity is 1% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta N176 acetate + cephalosporin C
-
 ?
3.5.1.93 succinyl-7-aminocephalosporanic acid + H2O activity is 32% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta 7-aminocephalosporanate + succinate
-
 ?
3.5.1.93 succinyl-7-aminocephalosporanic acid + H2O activity is 32% of the activity with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta N176 7-aminocephalosporanate + succinate
-
 ?
3.5.1.93 succinyl-7-aminocephalosporanic acid + H2O activity is 6.1% of the activity with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus 7-aminocephalosporanic acid + succinate
-
 ?
3.5.1.93 succinyl-7-aminocephalosporanic acid + H2O activity is 9.9fold higher than with glutaryl-7-aminocephalosporanic acid Pseudomonas sp. 7-aminocephalosporanic acid + succinate
-
 ?
3.5.1.93 succinyl-7-aminocephalosporanic acid + H2O activity is 6.1% of the activity with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus J1 7-aminocephalosporanic acid + succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.93 ? 1 * 70000, SDS-PAGE Brevibacillus laterosporus
3.5.1.93 ? 1 * 26000 + 1 * 58000, SDS-PAGE Brevundimonas diminuta
3.5.1.93 dimer 1 * 28000 + 1 * 61000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 cephalosporin C acylase
-
 Pseudomonas sp.
3.5.1.93 cephalosporin C acylase
-
 Brevibacillus laterosporus
3.5.1.93 cephalosporin C acylase
-
 Brevundimonas diminuta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 40
-
-
 Brevibacillus laterosporus
3.5.1.93 40
-
-
 Brevundimonas diminuta
3.5.1.93 48
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.93 30 55 30°C: about 45% of maximal activity, 55°C: about 70% of maximal activity Pseudomonas sp.
3.5.1.93 30 55 30°C: about 45% of maximal activity, 55°C: about 70% of maximal activity Brevibacillus laterosporus
3.5.1.93 30 55 30°C: about 45% of maximal activity, 55°C: about 70% of maximal activity Brevundimonas diminuta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.93 50
-
 1 h, less than 10% loss of activity at pH 6.0, about 60% less of activity at pH 5, about 35% loss of activity at pH 7, about 50% loss of activity at pH 8, about 90% loss of activity at pH 9 Pseudomonas sp.
3.5.1.93 50
-
 1 h, less than 10% loss of activity at pH 7.0, about 20% less of activity at pH 5, about 35% loss of activity at pH 7, about 70% loss of activity at pH 8, about 90% loss of activity at pH 9 Brevibacillus laterosporus
3.5.1.93 50
-
 1 h, stable at pH 9.0, about 45% less of activity at pH 6, about 15% loss of activity at pH 7, about 10% loss of activity at pH 8, about 60% loss of activity at pH 10 Brevundimonas diminuta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 8
-
 reaction with glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
3.5.1.93 9
-
 reaction with glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta
3.5.1.93 9 10 reaction with glutaryl-7-aminocephalosporanic acid Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.93 6 9 pH 6: about 50% of maximal activity, pH 90: about 90% of maximal activity, hydrolysis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
3.5.1.93 7 9 activity decrease from pH 7 to pH 9, synthesis of glutaryl-7-aminocephalosporanic acid Pseudomonas sp.
3.5.1.93 7 9 activity decreases from pH 7 to pH 9, synthesis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
3.5.1.93 8 10 pH 8: about 50% of maximal activity, pH 9-10: maximal activity, hydrolysis of glutaryl-7-aminocephalosporanic acid Pseudomonas sp.
3.5.1.93 8
-
 activity decrease from pH 7 to pH 9, synthesis of glutaryl-7-aminocephalosporanic acid Brevundimonas diminuta

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.93 1.4
-
 7-aminocephalosporanate
-
 Brevundimonas diminuta
3.5.1.93 2
-
 7-aminocephalosporanate
-
 Brevibacillus laterosporus
3.5.1.93 2.3
-
 7-aminocephalosporanate
-
 Pseudomonas sp.
3.5.1.93 2.5
-
 Glutarate
-
 Brevundimonas diminuta
3.5.1.93 10
-
 Glutarate
-
 Pseudomonas sp.
3.5.1.93 53
-
 Glutarate
-
 Brevibacillus laterosporus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.93 Brevundimonas diminuta isoelectric focusing
-
 4.5
3.5.1.93 Brevibacillus laterosporus isoelectric focusing
-
 4.7
3.5.1.93 Pseudomonas sp. above, isoelectric focusing
-
 6.5