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Literature summary extracted from

  • Ju, J.; Yokoigawa, K.; Misono, H.; Ohnishi, K.
    Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of gene products expressed in Escherichia coli (2005), J. Biosci. Bioeng., 100, 409-417.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 strain has one single enzyme gene, expression in Escherichia coli with His-tag Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 4.7
-
D-alanine pH 7.4, 30°C Pseudomonas fluorescens
5.1.1.1 8.1
-
L-alanine pH 7.4, 30°C Pseudomonas fluorescens
5.1.1.1 8.7
-
D-alanine pH 7.4, 30°C Pseudomonas fluorescens
5.1.1.1 18.4
-
L-alanine pH 7.4, 30°C Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 39000
-
1 * 39000, SDS-PAGE, 1 * 39700, calculated, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 39000
-
1 * 39000, SDS-PAGE, 1 * 39800, calculated, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 39000
-
2 * 39000, SDS-PAGE, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 39700
-
1 * 39000, SDS-PAGE, 1 * 39700, calculated, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 39800
-
1 * 39000, SDS-PAGE, 1 * 39800, calculated, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 40000
-
gel filtration Pseudomonas fluorescens
5.1.1.1 80000
-
gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Pseudomonas fluorescens
-
-
-
5.1.1.1 Pseudomonas fluorescens
-
soil-borne strain LRB3W1
-
5.1.1.1 Pseudomonas fluorescens TM5-2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 D-alanine
-
Pseudomonas fluorescens L-alanine
-
r
5.1.1.1 D-alanine
-
Pseudomonas fluorescens TM5-2 L-alanine
-
r
5.1.1.1 L-alanine
-
Pseudomonas fluorescens D-alanine
-
r
5.1.1.1 L-alanine
-
Pseudomonas fluorescens TM5-2 D-alanine
-
r

Subunits

EC Number Subunits Comment Organism
5.1.1.1 dimer 2 * 39000, SDS-PAGE, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 monomer 1 * 39000, SDS-PAGE, 1 * 39700, calculated, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens
5.1.1.1 monomer 1 * 39000, SDS-PAGE, 1 * 39800, calculated, enzyme shows monomer-dimer equilibrium Pseudomonas fluorescens