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Literature summary extracted from

  • Jiang, H.; Rao, K.S.; Yee, V.C.; Kraus, J.P.
    Characterization of four variant forms of human propionyl-CoA carboxylase expressed in Escherichia coli (2005), J. Biol. Chem., 280, 27719-27727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.3 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.4.1.3 A497V polymorphism, 30% higher specific activity than wild-type enzyme Homo sapiens
6.4.1.3 E168K mutant enzyme shows 32.3% of the specific activity of purified wild-type enzyme Homo sapiens
6.4.1.3 R165W mutant enzyme shows 42.5% of the specific activity of purified wild-type enzyme Homo sapiens
6.4.1.3 R410W mutant enzyme shows 42.61% of the specific activity of purified wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.3 0.28
-
propanoyl-CoA 37°C, pH 8.0, mutant enzyme E168K Homo sapiens
6.4.1.3 0.33
-
propanoyl-CoA 37°C, pH 8.0, mutant enzyme R165W Homo sapiens
6.4.1.3 0.41
-
propanoyl-CoA 37°C, pH 8.0, wild-type enzyme Homo sapiens
6.4.1.3 0.54
-
propanoyl-CoA 37°C, pH 8.0, mutant enzyme R410W Homo sapiens
6.4.1.3 0.59
-
propanoyl-CoA 37°C, pH 8.0, mutant enzyme A497V Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.3 800000
-
gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.3 recombinant Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.3 7.54
-
purified mutant enzyme E168K Homo sapiens
6.4.1.3 9.93
-
purified mutant enzyme R165W Homo sapiens
6.4.1.3 9.95
-
purified mutant enzyme R410W Homo sapiens
6.4.1.3 23.35
-
purified wild-type enzyme Homo sapiens
6.4.1.3 32.34
-
purified mutant enzyme A497V Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
Homo sapiens ADP + phosphate + (S)-methylmalonyl-CoA
-
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Subunits

EC Number Subunits Comment Organism
6.4.1.3 dodecamer alpha6beta6 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.4.1.3 PCC
-
Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.3 additional information
-
thermal denaturation is irreversible Homo sapiens
6.4.1.3 37
-
30 min, wild-type enzyme is stable, mutant enzymes R165W, E168K and R410W lose 30% of their activity Homo sapiens
6.4.1.3 47
-
30 min, wild-type enzyme is stable, mutant enzyme A497V loses 40% of its activity mutant enzymes R165W, E168K and R410W lose 85% of their activity Homo sapiens
6.4.1.3 58
-
the wild-type enzyme undergoes a cooperative two-state transition between the native and denatured states with a Tm of 57.6°C Homo sapiens