EC Number | Cloned (Comment) | Organism |
---|---|---|
6.4.1.3 | expression in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.4.1.3 | A497V | polymorphism, 30% higher specific activity than wild-type enzyme | Homo sapiens |
6.4.1.3 | E168K | mutant enzyme shows 32.3% of the specific activity of purified wild-type enzyme | Homo sapiens |
6.4.1.3 | R165W | mutant enzyme shows 42.5% of the specific activity of purified wild-type enzyme | Homo sapiens |
6.4.1.3 | R410W | mutant enzyme shows 42.61% of the specific activity of purified wild-type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.3 | 0.28 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme E168K | Homo sapiens | |
6.4.1.3 | 0.33 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme R165W | Homo sapiens | |
6.4.1.3 | 0.41 | - |
propanoyl-CoA | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
6.4.1.3 | 0.54 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme R410W | Homo sapiens | |
6.4.1.3 | 0.59 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme A497V | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | 800000 | - |
gel filtration | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.3 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.3 | recombinant | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | 7.54 | - |
purified mutant enzyme E168K | Homo sapiens |
6.4.1.3 | 9.93 | - |
purified mutant enzyme R165W | Homo sapiens |
6.4.1.3 | 9.95 | - |
purified mutant enzyme R410W | Homo sapiens |
6.4.1.3 | 23.35 | - |
purified wild-type enzyme | Homo sapiens |
6.4.1.3 | 32.34 | - |
purified mutant enzyme A497V | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Homo sapiens | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.3 | dodecamer | alpha6beta6 | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.4.1.3 | PCC | - |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.3 | additional information | - |
thermal denaturation is irreversible | Homo sapiens |
6.4.1.3 | 37 | - |
30 min, wild-type enzyme is stable, mutant enzymes R165W, E168K and R410W lose 30% of their activity | Homo sapiens |
6.4.1.3 | 47 | - |
30 min, wild-type enzyme is stable, mutant enzyme A497V loses 40% of its activity mutant enzymes R165W, E168K and R410W lose 85% of their activity | Homo sapiens |
6.4.1.3 | 58 | - |
the wild-type enzyme undergoes a cooperative two-state transition between the native and denatured states with a Tm of 57.6°C | Homo sapiens |