EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.7 | mutant proteins expressed from baculovirus constructs in insect cells | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.7 | DELTA413470 | mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity | Drosophila melanogaster |
2.7.7.7 | DELTA483533 | expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant holoenzyme is reduced greatly as compared to wild type | Drosophila melanogaster |
2.7.7.7 | DELTA536581 | expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant enzyme is 80% of wild-type holoenzyme | Drosophila melanogaster |
2.7.7.7 | DELTA666742 | mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity | Drosophila melanogaster |
2.7.7.7 | F576A | mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity | Drosophila melanogaster |
2.7.7.7 | G575A | mutation in alpha-subunit, mutant enzyme has nearly normal activity | Drosophila melanogaster |
2.7.7.7 | G575A/W576A/F578A | mutation in alpha-subunit completely reduces DNA polymerase activity | Drosophila melanogaster |
2.7.7.7 | K557A | mutation in alpha-subunit, mutant enzyme has nearly normal activity | Drosophila melanogaster |
2.7.7.7 | K687A/D688A/F689A | mutation in alpha-subunit resulted in DNA polymerase activities that is 6080% of wild-type enzyme | Drosophila melanogaster |
2.7.7.7 | L558A | mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity | Drosophila melanogaster |
2.7.7.7 | P556A | mutation in alpha-subunit, mutant enzyme has nearly normal activity | Drosophila melanogaster |
2.7.7.7 | P556A/K557A/L558A | mutation in alpha-subunit completely reduces DNA polymerase activity | Drosophila melanogaster |
2.7.7.7 | S719A/Y720A/W721 | mutation in alpha-subunit completely reduces DNA polymerase activity | Drosophila melanogaster |
2.7.7.7 | W576A | mutation in alpha-subunit, mutant enzyme is nearly inactive | Drosophila melanogaster |
2.7.7.7 | Y419A/E420A/D421A | mutation in alpha-subunit resulted in DNA polymerase activities that is 6080% of wild-type enzyme | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.7 | mitochondrion | - |
Drosophila melanogaster | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.7 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.7 | recombinant mutant proteins | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.7 | deoxynucleoside triphosphate + DNAn | - |
Drosophila melanogaster | diphosphate + DNAn+1 | - |
? |