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Literature summary extracted from

  • Luo, N.; Kaguni, L.S.
    Mutations in the spacer region of Drosophila mitochondrial DNA polymerase affect DNA binding, processivity, and the balance between Pol and Exo function (2005), J. Biol. Chem., 280, 2491-2497.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 mutant proteins expressed from baculovirus constructs in insect cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 DELTA413–470 mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity Drosophila melanogaster
2.7.7.7 DELTA483–533 expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant holoenzyme is reduced greatly as compared to wild type Drosophila melanogaster
2.7.7.7 DELTA536–581 expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant enzyme is 80% of wild-type holoenzyme Drosophila melanogaster
2.7.7.7 DELTA666–742 mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity Drosophila melanogaster
2.7.7.7 F576A mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity Drosophila melanogaster
2.7.7.7 G575A mutation in alpha-subunit, mutant enzyme has nearly normal activity Drosophila melanogaster
2.7.7.7 G575A/W576A/F578A mutation in alpha-subunit completely reduces DNA polymerase activity Drosophila melanogaster
2.7.7.7 K557A mutation in alpha-subunit, mutant enzyme has nearly normal activity Drosophila melanogaster
2.7.7.7 K687A/D688A/F689A mutation in alpha-subunit resulted in DNA polymerase activities that is 60–80% of wild-type enzyme Drosophila melanogaster
2.7.7.7 L558A mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity Drosophila melanogaster
2.7.7.7 P556A mutation in alpha-subunit, mutant enzyme has nearly normal activity Drosophila melanogaster
2.7.7.7 P556A/K557A/L558A mutation in alpha-subunit completely reduces DNA polymerase activity Drosophila melanogaster
2.7.7.7 S719A/Y720A/W721 mutation in alpha-subunit completely reduces DNA polymerase activity Drosophila melanogaster
2.7.7.7 W576A mutation in alpha-subunit, mutant enzyme is nearly inactive Drosophila melanogaster
2.7.7.7 Y419A/E420A/D421A mutation in alpha-subunit resulted in DNA polymerase activities that is 60–80% of wild-type enzyme Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.7 mitochondrion
-
Drosophila melanogaster 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 recombinant mutant proteins Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
Drosophila melanogaster diphosphate + DNAn+1
-
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