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Literature summary extracted from

  • Bouhss, A.; Crouvoisier, M.; Blanot, D.; Mengin-Lecreulx, D.
    Purification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis (2004), J. Biol. Chem., 279, 29974-29980.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.13 additional information N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.13 His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Staphylococcus aureus
2.7.8.13 His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Bacillus subtilis
2.7.8.13 His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Escherichia coli
2.7.8.13 His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.13 additional information N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity Bacillus subtilis
2.7.8.13 tunicamycin IC50: 0.012 mM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.13 0.16
-
 undecaprenyl phosphate
-
 Bacillus subtilis
2.7.8.13 1
-
 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.13 membrane integral membrane protein Bacillus subtilis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.13 Mg2+ absolute requirement for divalent cations, optimal activity with Mg2+ at concentrations 40-75 mM Bacillus subtilis
2.7.8.13 Mn2+ enzyme activity in presence of Mn2+ is 1% of the activity detected in presence of Mg2+ Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Bacillus subtilis catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Escherichia coli catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Thermotoga maritima catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Staphylococcus aureus catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.13 Bacillus subtilis
-
 recombinant
-
2.7.8.13 Escherichia coli
-
 recombinant
-
2.7.8.13 Staphylococcus aureus
-
 recombinant
-
2.7.8.13 Thermotoga maritima
-
 recombinant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.13
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.13 additional information
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Bacillus subtilis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Escherichia coli UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Thermotoga maritima UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Bacillus subtilis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Escherichia coli UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Thermotoga maritima UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Staphylococcus aureus UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
2.7.8.13 UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Staphylococcus aureus UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.8.13 MraY translocase
-
 Staphylococcus aureus
2.7.8.13 MraY translocase
-
 Bacillus subtilis
2.7.8.13 MraY translocase
-
 Escherichia coli
2.7.8.13 MraY translocase
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.13 5.33
-
 undecaprenyl phosphate
-
 Bacillus subtilis
2.7.8.13 5.33
-
 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
 Bacillus subtilis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.8.13 0.012
-
 IC50: 0.012 mM Bacillus subtilis tunicamycin