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Literature summary extracted from

  • Gong, C.; Martins, A.; Bongiorno, P.; Glickman, M.; Shuman, S.
    Biochemical and genetic analysis of the four DNA ligases of mycobacteria (2004), J. Biol. Chem., 279, 20594-20606.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 ATP-dependent ligase LigB is produced in Escherichia coli as a His10-LigB fusion protein Mycobacterium tuberculosis
6.5.1.1 ATP-dependent ligase LigC is produced in Escherichia coli as a His10-LigC fusion protein Mycobacterium tuberculosis
6.5.1.1 ATP-dependent ligase LigD is produced in Escherichia coli as a His10-LigD fusion protein Mycobacterium tuberculosis
6.5.1.2 produced in Escherichia coli as a His10-LigA fusion Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 pyridochromanone IC50: 0.0006 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.34
-
 ATP ATP-dependent ligase LigB Mycobacterium tuberculosis
6.5.1.2 0.0013
-
 NAD+
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis ATP-dependent ligase LigB displays vigorous nick sealing activity in presence of NAD+ and ATP AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis ATP-dependent ligase LigC displays weak nick joining activity and generates high levels of DNA adenylate intermediate AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis ATP-dependent ligase LigD displays weak nick joining activity and generates high levels of DNA adenylate intermediate AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis LigA is essential for growth of Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Mycobacterium tuberculosis
-
 ATP-dependent ligase LigB
-
6.5.1.1 Mycobacterium tuberculosis
-
 ATP-dependent ligase LigC
-
6.5.1.1 Mycobacterium tuberculosis
-
 ATP-dependent ligase LigD
-
6.5.1.2 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 His10-LigB fusion protein expressed in Escherichia coli Mycobacterium tuberculosis
6.5.1.1 His10-LigC fusion protein expressed in Escherichia coli Mycobacterium tuberculosis
6.5.1.1 His10-LigD fusion protein expressed in Escherichia coli Mycobacterium tuberculosis
6.5.1.2 His10-LigA fusion Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP-dependent ligase LigB displays vigorous nick sealing activity in presence of NAD+ and ATP Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP-dependent ligase LigC displays weak nick joining activity and generates high levels of DNA adenylate intermediate Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP-dependent ligase LigD displays weak nick joining activity and generates high levels of DNA adenylate intermediate Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m LigA is essential for growth of Mycobacterium tuberculosis Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer ATP-dependent ligase LigB Mycobacterium tuberculosis
6.5.1.1 monomer ATP-dependent ligase LigC Mycobacterium tuberculosis
6.5.1.1 monomer ATP-dependent ligase LigD Mycobacterium tuberculosis
6.5.1.2 monomer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent ligase LigB
-
 Mycobacterium tuberculosis
6.5.1.1 ATP-dependent ligase LigC
-
 Mycobacterium tuberculosis
6.5.1.1 ATP-dependent ligase LigD
-
 Mycobacterium tuberculosis
6.5.1.1 LigB
-
 Mycobacterium tuberculosis
6.5.1.1 LigC
-
 Mycobacterium tuberculosis
6.5.1.1 LigD
-
 Mycobacterium tuberculosis
6.5.1.1 MtuLigB
-
 Mycobacterium tuberculosis
6.5.1.1 MtuLigC
-
 Mycobacterium tuberculosis
6.5.1.1 MtuLigD
-
 Mycobacterium tuberculosis
6.5.1.2 LigA
-
 Mycobacterium tuberculosis
6.5.1.2 MtuLigA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.2 0.072
-
 NAD+
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+
-
 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.5.1.2 0.0006
-
 IC50: 0.0006 mM Mycobacterium tuberculosis pyridochromanone