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Literature summary extracted from

  • Choi, J.Y.; Guengerich, F.P.
    Analysis of the effect of bulk at N2-alkylguanine DNA adducts on catalytic efficiency and fidelity of the processive DNA polymerases bacteriophage T7 exonuclease- and HIV-1 reverse transcriptase (2004), J. Biol. Chem., 279, 19217-19229.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.49 N2-benzylguanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1
2.7.7.49 N2-ethylguanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1
2.7.7.49 N2-isobutylguanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1
2.7.7.49 N2-methyl(9-anthracenyl)guanine the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity Human immunodeficiency virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.49 0.00002
-
 dCTP
-
 Human immunodeficiency virus 1
2.7.7.49 0.25
-
 dTTP
-
 Human immunodeficiency virus 1
2.7.7.49 0.85
-
 dATP
-
 Human immunodeficiency virus 1

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.49 Human immunodeficiency virus 1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.49 dCTP + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 deoxynucleoside triphosphate + DNAn
-
 Human immunodeficiency virus 1 diphosphate + DNAn+1
-
 ?
2.7.7.49 dTTP + poly(rA)/(dT)18
-
 Human immunodeficiency virus 1 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.49 additional information
-
 additional information
-
 Human immunodeficiency virus 1