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Literature summary extracted from

  • Eichmann, T.; Lorenz, K.; Hoffmann, M.; Brockmann, J.; Krasel, C.; Lohse, M.J.; Quitterer, U.
    The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gbeta gamma binding site (2003), J. Biol. Chem., 278, 8052-8057.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 G protein Gbetagamma subunits required for activity, 20fold stimulation with substrate rhodopsin, 2 regulatory Gbetagamma binding sites: one N-terminal domain of GRK2, and one C-terminal within the pleckstrin homology domain, both sites are functionally different, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 expression of GST-tagged wild-type and truncation mutant GRK2s in Escherichia coli, expression of wild-type GRK2 in Spodoptera frugiperda Sf9 cells using the baculovirus infection system, expression of wild-type and truncation mutant GRK2s in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.15 additional information construction of deletion mutants comprising residues 1-485, 1-185, 561-689, 1-53, 1-200, or 54-185, analysis of activity with rhodopsin and binding of G protein betagamma subunits, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.15 additional information deletion mutants of the enzyme, comprising parts of the C-terminus or the N-terminus, are able to inhibit the full-length wild-type enzyme, overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant GST-tagged wild-type and truncation mutant GRK2s from Escherichia coli by glutathione affinity chromatography, recombinant wild-type GRK2 from Sf9 insect cells by heparin affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + rhodopsin
-
 Homo sapiens ADP + phosphorylated rhodopsin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.15 G protein-coupled receptor kinase 2
-
 Homo sapiens
2.7.11.15 GRK2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 22
-
 assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Homo sapiens