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Literature summary extracted from

  • Billich, A.; Bornancin, F.; Devay, P.; Mechtcheriakova, D.; Urtz, N.; Baumruker, T.
    Phosphorylation of the immunomodulatory drug FTY720 by sphingosine kinases (2003), J. Biol. Chem., 278, 47408-47415.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91 isozymes SPHK1 and SPHK2, expression in HEK293 cells as EGFP-fusion proteins Mus musculus
2.7.1.91 isozymes SPHK1 and SPHK2, several splicing variants are differently active, expression in HEK293 cells as EGFP-fusion proteins Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.91 additional information
-
 additional information kinetics, isozymes SPHK1 and SPHK2 Homo sapiens
2.7.1.91 additional information
-
 additional information kinetics, isozymes SPHK1 and SPHK2 Mus musculus
2.7.1.91 0.013
-
 sphingosine pH 7.4, 30°C, recombinant isozyme SPHK1a Mus musculus
2.7.1.91 0.013
-
 sphingosine pH 7.4, 30°C, recombinant isozyme SPHK2 Mus musculus
2.7.1.91 0.014
-
 sphingosine pH 7.4, 30°C, recombinant isozyme SPHK2 Homo sapiens
2.7.1.91 0.016
-
 sphingosine pH 7.4, 30°C, recombinant isozyme SPHK1 Homo sapiens
2.7.1.91 0.018
-
 FTY720 pH 7.4, 30°C, recombinant isozyme SPHK2 Homo sapiens
2.7.1.91 0.024
-
 FTY720 pH 7.4, 30°C, recombinant isozyme SPHK2 Mus musculus
2.7.1.91 0.692
-
 FTY720 about, pH 7.4, 30°C, recombinant isozyme SPHK1a Mus musculus
2.7.1.91 0.785
-
 FTY720 about, pH 7.4, 30°C, recombinant isozyme SPHK1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.91 cytosol
-
 Homo sapiens 5829
-
2.7.1.91 cytosol major part of isozyme SPHK1 and isozyme SPHK2 Mus musculus 5829
-
2.7.1.91 particle-bound
-
 Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.91 ATP + sphinganine Homo sapiens
-
 ADP + sphinganine 1-phosphate
-
 ?
2.7.1.91 ATP + sphinganine Mus musculus
-
 ADP + sphinganine 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Homo sapiens Q9NRA0 isozyme SPHK2; 2 isozymes SPHK1 and SPHK2, several splicing variants
-
2.7.1.91 Homo sapiens Q9NYA1 isozyme SPHK1; 2 isozymes SPHK1 and SPHK2, several splicing variants
-
2.7.1.91 Mus musculus O88885 isozyme SPHK1; 2 isozymes SPHK1 and SPHK2, several splicing variants
-
2.7.1.91 Mus musculus Q9JIA7 isozyme SPHK2; 2 isozymes SPHK1 and SPHK2, several splicing variants
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 blood higher activity of isozyme SPHK1, low activity of isozyme SPHK2 Homo sapiens
-
2.7.1.91 blood higher activity of isozyme SPHK1, low activity of isozyme SPHK2 Mus musculus
-
2.7.1.91 brain quite low activity of isozyme SPHK1 and isozyme SPHK2 Mus musculus
-
2.7.1.91 endothelial cell
-
 Homo sapiens
-
2.7.1.91 kidney isozyme SPHK1 and isozyme SPHK2 Mus musculus
-
2.7.1.91 liver high activity of isozyme SPHK2, low activity of isozyme SPHK1 Mus musculus
-
2.7.1.91 lung high activity of isozyme SPHK1, low activity of isozyme SPHK2 Mus musculus
-
2.7.1.91 lymph node low activity of isozyme SPHK1 and isozyme SPHK2 Mus musculus
-
2.7.1.91 additional information tissue distribution of isozyme SPHK1 and isozyme SPHK2 expression and activity, overview Mus musculus
-
2.7.1.91 spleen isozyme SPHK1, low activity of isozyme SPHK2 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.91 additional information
-
 Vmax of different splicing variants of both isozymes Homo sapiens
2.7.1.91 additional information
-
 Vmax of different splicing variants of both isozymes Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + FTY720 immunomodulatory drug, high activity with isozyme SPHK2, 7fold lower activity with isozyme SPHK1 Homo sapiens ADP + FTY720 1-phosphate
-
 ?
2.7.1.91 ATP + FTY720 immunomodulatory drug, high activity with isozyme SPHK2, lower activity with isozyme SPHK1 Mus musculus ADP + FTY720 1-phosphate
-
 ?
2.7.1.91 ATP + sphinganine
-
 Homo sapiens ADP + sphinganine 1-phosphate
-
 ?
2.7.1.91 ATP + sphinganine
-
 Mus musculus ADP + sphinganine 1-phosphate
-
 ?
2.7.1.91 ATP + sphinganine 2fold higher activity with isozyme SPHK1 compared to isozyme SPHK2 Homo sapiens ADP + sphinganine 1-phosphate
-
 ?
2.7.1.91 ATP + sphingosine
-
 Mus musculus ADP + sphingosine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 More enzyme belongs to the SPHK superfamily Homo sapiens
2.7.1.91 More enzyme belongs to the SPHK superfamily Mus musculus
2.7.1.91 sphinganine kinase
-
 Homo sapiens
2.7.1.91 sphinganine kinase
-
 Mus musculus
2.7.1.91 SPHK
-
 Homo sapiens
2.7.1.91 SPHK
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.91 30
-
 assay at Homo sapiens
2.7.1.91 30
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 7.4
-
 assay at Homo sapiens
2.7.1.91 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.91 ATP
-
 Mus musculus
2.7.1.91 ATP binding site contain the GGDGX20K motif Homo sapiens
2.7.1.91 ATP binding site contains the GGDGX20K motif Homo sapiens