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Literature summary extracted from

  • Johnsen, U.; Hansen, T.; Schoenheit, P.
    Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima. Unusual regulatory properties in hyperthermophilic archaea (2003), J. Biol. Chem., 278, 25417-25427.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 AMP allosteric activator Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40
-
 Pyrobaculum aerophilum
2.7.1.40
-
 Aeropyrum pernix
2.7.1.40 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ATP allosteric inhibitor Thermotoga maritima
2.7.1.40 KCl 40 mM, 50-60% decrease in activity of recombinant enzyme Thermotoga maritima
2.7.1.40 NH4Cl 40 mM, 50-60% decrease in activity of recombinant enzyme Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Ca2+ divalent cation required, at 1 mM Ca2+ activation results in 14% of the activity with Mg2+ Aeropyrum pernix
2.7.1.40 Ca2+ divalent cation required, at 1 mM Ca2+ activation results in 3% of the activity with Mg2+ Thermotoga maritima
2.7.1.40 Ca2+ divalent cation required, at 1 mM Ca2+ activation results in 7% of the activity with Mg2+ Archaeoglobus fulgidus
2.7.1.40 Co2+ divalent cation required, at 1 mM Co2+ activation results in 120% of the activity with Mg2+ Thermotoga maritima
2.7.1.40 Co2+ divalent cation required, at 1 mM Co2+ activation results in 170% of the activity with Mg2+ Aeropyrum pernix
2.7.1.40 Co2+ divalent cation required, at 1 mM Co2+ activation results in 80% of the activity with Mn2+ Pyrobaculum aerophilum
2.7.1.40 Cu2+ divalent cation required, at 1 mM Cu2+ activation results in 86% of the activity with Mg2+ Archaeoglobus fulgidus
2.7.1.40 Mg2+ divalent cation required Thermotoga maritima
2.7.1.40 Mg2+ divalent cation required Aeropyrum pernix
2.7.1.40 Mg2+ divalent cation required, at 1 mM highest activity with Mg2+ Archaeoglobus fulgidus
2.7.1.40 Mn2+ divalent cation required Pyrobaculum aerophilum
2.7.1.40 Mn2+ divalent cation required, at 1 mM Mn2+ activation results in 160% of the activity with Mg2+ Aeropyrum pernix
2.7.1.40 Mn2+ divalent cation required, at 1 mM Mn2+ activation results in 35% of the activity with Mg2+ Thermotoga maritima
2.7.1.40 Mn2+ divalent cation required, at 1 mM Mn2+ activation results in 63% of the activity with Mg2+ Archaeoglobus fulgidus
2.7.1.40 additional information 1 mM Mg2+ does not shows significant activity. No activity is observed with Ca2+ and Zn2+ Thermotoga maritima
2.7.1.40 additional information 1 mM Mg2+ does not shows significant activity. No activity is observed with Ca2+ and Zn2+ Pyrobaculum aerophilum
2.7.1.40 Ni2+ divalent cation required, at 1 mM Ni2+ activation results in 2% of the activity with Mg2+ Archaeoglobus fulgidus
2.7.1.40 Zn2+ divalent cation required, at 1 mM Zn2 activation results in 2.5% of the activity with Mg2+ Thermotoga maritima
2.7.1.40 Zn2+ divalent cation required, at 1 mM Zn2+ activation results in 11% of the activity with Mg2+ Aeropyrum pernix
2.7.1.40 Zn2+ divalent cation required, at 1 mM Zn2+ activation results in 6.5% of the activity with Mg2+ Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 48000
-
 4 * 48000, SDS-PAGE Pyrobaculum aerophilum
2.7.1.40 49000
-
 4 * 49000, SDS-PAGE Archaeoglobus fulgidus
2.7.1.40 51000
-
 4 * 51000, SDS-PAGE Aeropyrum pernix
2.7.1.40 51000
-
 4 * 51000, native enzyme, SDS-PAGE Thermotoga maritima
2.7.1.40 56000
-
 4 * 56000, recombinant enzyme, SDS-PAGE Thermotoga maritima
2.7.1.40 190000
-
 recombinant enzyme, gel filtration Thermotoga maritima
2.7.1.40 194000
-
 native enzyme, gel filtration Thermotoga maritima
2.7.1.40 203000
-
 gel filtration Archaeoglobus fulgidus
2.7.1.40 205000
-
 gel filtration Pyrobaculum aerophilum
2.7.1.40 207000
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Aeropyrum pernix
-
-
-
2.7.1.40 Archaeoglobus fulgidus
-
-
-
2.7.1.40 Archaeoglobus fulgidus 7324
-
-
-
2.7.1.40 Pyrobaculum aerophilum
-
-
-
2.7.1.40 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Archaeoglobus fulgidus
2.7.1.40
-
 Thermotoga maritima
2.7.1.40
-
 Pyrobaculum aerophilum
2.7.1.40
-
 Aeropyrum pernix

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 320
-
-
 Thermotoga maritima
2.7.1.40 1000
-
 80°C Archaeoglobus fulgidus

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 51000, SDS-PAGE Aeropyrum pernix
2.7.1.40 tetramer 4 * 48000, SDS-PAGE Pyrobaculum aerophilum
2.7.1.40 tetramer 4 * 49000, SDS-PAGE Archaeoglobus fulgidus
2.7.1.40 tetramer 4 * 51000, native enzyme, SDS-PAGE Thermotoga maritima
2.7.1.40 tetramer 4 * 56000, recombinant enzyme, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 80
-
 recombinant enzyme Thermotoga maritima
2.7.1.40 85
-
-
 Archaeoglobus fulgidus
2.7.1.40 95
-
 above Aeropyrum pernix
2.7.1.40 98
-
 above Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 70
-
 120 min, stable Archaeoglobus fulgidus
2.7.1.40 90
-
 half-life: 20 min Archaeoglobus fulgidus
2.7.1.40 100
-
 half-life: 220 min Pyrobaculum aerophilum
2.7.1.40 100
-
 120 min, stable Aeropyrum pernix
2.7.1.40 100
-
 almost complete loss of activity after 7 min. Addition of 1 M (NH4)2SO4 effectively stabilizes the enzyme agianst heat inactivation, retaining about 50% residual activity after incubation at 120 min Archaeoglobus fulgidus
2.7.1.40 100
-
 half-life: about 20 min, almost complete loss of activity after 120 min, native and recombinant enzyme, about 40% residual activity after 120 min incubation in presence of 1 M (NH4)2SO4 Thermotoga maritima
2.7.1.40 110
-
 half-life: 30 min. (NH4)2SO4, NaCl or KCl, each at 1 mM, do not stabilize the enzyme agianst heat inactivation Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 5.9
-
 recombiannt enzyme Thermotoga maritima
2.7.1.40 6
-
-
 Pyrobaculum aerophilum
2.7.1.40 6
-
 native enzyme Thermotoga maritima
2.7.1.40 6.1
-
-
 Aeropyrum pernix
2.7.1.40 6.6
-
-
 Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 5.5 7 about 30% of maximal activity at pH 5.5 and 7.0 Thermotoga maritima
2.7.1.40 5.5 7.5 about 50% of maximal activity at pH 5.5 and pH 7.5 Archaeoglobus fulgidus
2.7.1.40 5.5 7.5 about 50% of maximal activity at pH 5.5 and pH 7.5 Aeropyrum pernix