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Literature summary extracted from

  • Bird, L.E.; Ren, J.; Wright, A.; Leslie, K.D.; Degreve, B.; Balzarini, J.; Stammers, D.K.
    Crystal structure of Varicella zoster virus thymidine kinase (2003), J. Biol. Chem., 278, 24680-24687.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expression as GST-tagged enzyme in Escherichia coli strain BL21 Human alphaherpesvirus 3

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.21 purified recombinant GST-tagged enzyme complexed with ADP and E-5-(2-bromovinyl)-2'-deoxythymidine, sitting drop vapour diffusion method, 21°C, mixing of 0.001 ml of enzyme and reservoir solution, the enzyme solution contains 8 mg/ml protein, 0.4 mM ADP, 0.2 mM E-5-(2-bromovinyl)-2'-deoxythymidine, and 0.6 mM MgCl2, reservoir solution contains 0.1 M sodium acetate, at either pH 4.5 or 5.0, and 6-11% w/v PEG 20000, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement method Human alphaherpesvirus 3

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine Human alphaherpesvirus 3 drug activation by phosphorylation ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine Human alphaherpesvirus 3
-
 ADP + thymidine 5'-phosphate
-
 ?
2.7.1.21 additional information Human alphaherpesvirus 3 the viral enzyme shows a broader substrate specificity than the human host cell enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Human alphaherpesvirus 3
-
 i.e. human Herpes virus 3, VZV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography Human alphaherpesvirus 3

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.21 ATP + thymidine = ADP + dTMP active site structure, substrate binding structures Human alphaherpesvirus 3
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
-
 Human alphaherpesvirus 3 ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine drug activation by phosphorylation Human alphaherpesvirus 3 ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine
-
 Human alphaherpesvirus 3 ADP + thymidine 5'-phosphate
-
 ?
2.7.1.21 additional information the viral enzyme shows a broader substrate specificity than the human host cell enzyme Human alphaherpesvirus 3 ?
-
 ?
2.7.1.21 additional information modeling of ganciclovir binding into the enzyme crystal structure, overview Human alphaherpesvirus 3 ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 ATP
-
 Human alphaherpesvirus 3