Literature summary extracted from
Ortiz-Lombardia, M.; Pompeo, F.; Boitel, B.; Alzari, P.M.
Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 13094-13100.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.11.1 |
expression of N-terminal catalytic domain of PknB, comprising residues 1-331, and of a construct comprising residues 1-279 in Escherichia coli BL21(DE3) as His-tagged proteins |
Mycobacterium tuberculosis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.11.1 |
purified recombinant His-tagged N-terminal catalytic domain of PknB, residues 1-331, hanging drop vapour diffusion method, 0.001 ml protein solution containing 5 mg/ml protein mixed with equal volume of 0.1 M HEPES, pH 7.5, 30 mM MgCl2, 0.15 mM inhibitor AMP-PNP, 27% PEG 400, and 4% 1,3-butanediol, 19°C, versus 1 ml of a solution containing 0.1 M HEPES, pH 7.5, 30 mM MgCl2, and 27% PEG 400, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution |
Mycobacterium tuberculosis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.11.1 |
AMP-PNP |
ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate |
Mycobacterium tuberculosis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.11.1 |
Mn2+ |
- |
Mycobacterium tuberculosis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.1 |
Mycobacterium tuberculosis |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.7.11.1 |
phosphoprotein |
PknB is autophosphorylated |
Mycobacterium tuberculosis |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.11.1 |
recombinant His-tagged N-terminal catalytic domain of PknB and His-tagged residues 1-279 construct from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
Mycobacterium tuberculosis |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.7.11.1 |
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate |
active site structure |
Mycobacterium tuberculosis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.11.1 |
ATP + myelic basic protein |
phosphorylation of serine and threonine residues by PknB |
Mycobacterium tuberculosis |
ADP + myelic basic phosphoprotein |
- |
? |
|
2.7.11.1 |
additional information |
PknB performs autophosphorylation |
Mycobacterium tuberculosis |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.11.1 |
PknB |
- |
Mycobacterium tuberculosis |
2.7.11.1 |
serine/threonine kinase |
- |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.7.11.1 |
22 |
- |
assay at room temperature |
Mycobacterium tuberculosis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.11.1 |
7 |
- |
assay at |
Mycobacterium tuberculosis |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.7.11.1 |
ATP |
- |
Mycobacterium tuberculosis |
|