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Literature summary extracted from

  • Ortiz-Lombardia, M.; Pompeo, F.; Boitel, B.; Alzari, P.M.
    Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 13094-13100.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expression of N-terminal catalytic domain of PknB, comprising residues 1-331, and of a construct comprising residues 1-279 in Escherichia coli BL21(DE3) as His-tagged proteins Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.11.1 purified recombinant His-tagged N-terminal catalytic domain of PknB, residues 1-331, hanging drop vapour diffusion method, 0.001 ml protein solution containing 5 mg/ml protein mixed with equal volume of 0.1 M HEPES, pH 7.5, 30 mM MgCl2, 0.15 mM inhibitor AMP-PNP, 27% PEG 400, and 4% 1,3-butanediol, 19°C, versus 1 ml of a solution containing 0.1 M HEPES, pH 7.5, 30 mM MgCl2, and 27% PEG 400, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 AMP-PNP ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mn2+
-
Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Mycobacterium tuberculosis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein PknB is autophosphorylated Mycobacterium tuberculosis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 recombinant His-tagged N-terminal catalytic domain of PknB and His-tagged residues 1-279 construct from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.1 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate active site structure Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + myelic basic protein phosphorylation of serine and threonine residues by PknB Mycobacterium tuberculosis ADP + myelic basic phosphoprotein
-
?
2.7.11.1 additional information PknB performs autophosphorylation Mycobacterium tuberculosis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PknB
-
Mycobacterium tuberculosis
2.7.11.1 serine/threonine kinase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 22
-
assay at room temperature Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7
-
assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
Mycobacterium tuberculosis